Immunofluorescence Assessment of TUBB3 Expression in Non-Small Cell Lung Cancer Tissue by Flow Cytometry: A 50% Marker Hyperexpression Threshold for Risk Stratification in Stage I Disease
Abstract An immunofluorescence analysis of TUBB3 expression was performed in tissue samples from patients with stage I and stage III non-small cell lung cancer (NSCLC, n = 65). A TUBB3 level of 50% demonstrated discriminatory ability for predicting disease outcomes, while ROC analysis confirmed that TUBB3 ≥50% can stratify patients with stage I NSCLC into a group with an aggressive disease course. Tumor TUBB3 hyperexpression ≥50% may serve as an additional criterion when selecting a management strategy for patients with stage I NSCLC who have undergone radical surgery, namely, adjuvant treatment or surveillance.
Authors
- Т. А. Богуш (ORCID: https://orcid.org/0000-0002-7673-4284)
- I. P. Romanov (ORCID: https://orcid.org/0000-0002-9931-1153)
- Ekaterina N. Kosobokova (ORCID: https://orcid.org/0000-0002-4660-8519)
- Maria A. Sventitskaya (ORCID: https://orcid.org/0009-0000-5766-0761)
- Anna N. Grishanina (ORCID: https://orcid.org/0000-0002-4277-9222)
- Mikhail Ya. Melnikov (ORCID: https://orcid.org/0000-0002-9712-8929)
- Alexander Mikhailovich Scherbakov (ORCID: https://orcid.org/0000-0002-2974-9555)
- Е. А. Богуш (ORCID: https://orcid.org/0000-0001-5601-3669)
Institutions
- Sechenov University (RU)
- Lomonosov Moscow State University (RU)
- Ministry of Health of the Russian Federation (RU)
Publication Details
- Journal
- Moscow University Chemistry Bulletin
- Published
- 2026-10-26
- DOI
- https://doi.org/10.3103/s0027131426060027
- Primary Topic
- Lung Cancer Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00