Detection of staphylococcus aureus as biofilm association with interleukin-1β among dental caries

Staphylococcus aureus is important in dental caries duo to biofilm formation have role in the dental caries inflammation, Bacterial antigens stimulate production of pro-inflammatory cytokine. This led to the hypothesis that in dental caries where B cells and plasma cells predomenate as a control, cytokines elicited by antigens from S. aureus, which frequently could play a significant role in eliciting the initial T-cell response. Furthermore, the oral saliva cytokine content was S. aureus as dental caries infection produced a significant level of numerous inflammatory cytokines. A total of 80 samples 50 samples (male: 31 and female: 19) suffering from dental caries and 30 sample (male: 13 males and 17: females), aged (20–60) years. During the acute stage of caries, samples are collected from patients attending to Department conservative in Dentistry College Babylon University period from November 2024 to February 2025 isolation by the sterile wooden swabs is put into transport media and then streaked Blood agar and semi selective Mannitol agar bacteria samples grown in aerobic incubation by spent 24 hours at 37°C S. aureus were isolated and defined by standard microbiological techniques and confirmed by VITEK-2 Compact system. S. aureus detection was Distribution of infection according to gender is (62%) male and (38%) female, biofilm formation ability by inoculating tubes containing 5 ml nutritional broth with young bacterial isolates. After the bacterial growth had been eliminated, each tube was stained by sefranin for 10 minutes. The stain was removed from the tubes and allowed to dry in the open air for 15 minutes; the staining of tube walls with a red color was regarded a positive outcome in biofilm test dental caries show that the prevalence of Biofilm procedure isolated bacteria was (90%) that biofilm formation, Interleukin-1β is (149.386 ± 13.12 ) Among Patients was find High significant compared to the controls (8.39 ± 0.289 pg/ml). The study findings that a high frequency of dental caries is associated with S aureus also this pathogen highly Biofilm-producing, and there is a higher significant level of IL-1β in patients than in control groups. IL-1β level. increase associated among dental caries patients.