Enhancing Biogas Production from Co-digestion of Organic Wastes Mixed with Cattle Manure Using Rumen Fluid and Saccharomyces Cerevisiae Isolate MUTJ0F

Background Biogas production from various types of biodegradable waste is a cost-effective, environmentally friendly, and sustainable alternative to fossil fuels, and provides a reliable method for handling food waste. Methods This study evaluated the effects of rumen fluid and S. cerevisiae isolate MUTJ0F as digestion activators on biogas production from the co-digestion of cattle manure, fruit and vegetable waste, and cafeteria leftover food. Under mesophilic conditions (38°C), 12 treatments with different rumen fluid (0–150 mL/100 g) and S. cerevisiae isolate MUTJ0F inoculum (5–10 mL/100 g) dosages were assessed for biogas production from mixed waste using plastic digesters (0.6 L) after a 60 days retention period. Result Rumen fluid with S. cerevisiae isolate MUTJ0F increased biogas generation by more than four times compared to the uninoculated control. The combination of 100 mL rumen fluid and 10 mL S. cerevisiae isolate MUTJ0F per 100 g of mixed waste produced the highest biogas yield (291.15 mL/gVS). pH variations in digesters revealed normal acidogenesis followed by a gradual return to methanogenesis conditions, while combustibility tests showed the presence of biogas in the digester. Conclusion These results suggest that using rumen fluid and the S. cerevisiae isolate MUTJ0F as inoculants in a biogas digester can enhance the efficiency of biogas production.