Repertoire diversity and dynamics of peripheral Th17 after allogeneic haematopoietic stem cell transplantation
Th17, a subset of helper T cells that produce interleukin-17 (IL-17), is thought to play a pivotal role in the pathogenesis of chronic graft-versus-host disease (GVHD). However, the kinetics of Th17 following allogeneic haematopoietic stem cell transplantation (allo-HSCT) have not been elucidated. In this study, we evaluated the temporal dynamics and diversity of peripherally circulating Th17 after allo-HSCT. The study included 86 patients who survived without disease relapse for 1 year or more, and for whom peripheral blood samples 3 months after allo-HSCT were available. Th17 was detected and sorted by flow cytometry, and T-cell receptor (TCR) repertoire was analysed by a next-generation sequencing (NGS) method. Patients with chronic GVHD showed a higher TCR repertoire diversity, while those with severe chronic GVHD showed a lower TCR diversity and Th17/Cluster of Differentiation (CD) 4 ratio. The amino acid sequence in complementarity determining region 3 of Th17-TCR was not apparently different between patients with and without chronic GVHD. Additionally, a higher Th17/CD4 ratio at 3 months was related to a lower incidence of subsequent chronic GVHD. The current study explored the kinetics of peripherally circulating Th17 after allo-HSCT, which might be associated with the development of chronic GVHD.
Authors
- Masakatsu Kawamura
- Masaharu Tamaki (ORCID: https://orcid.org/0000-0001-5899-7913)
- Akari Matsuoka (ORCID: https://orcid.org/0009-0000-3982-2253)
- Yuhei Nakamura (ORCID: https://orcid.org/0000-0002-3529-5676)
- Yoshinobu Kanda (ORCID: https://orcid.org/0000-0002-4866-9307)
- Yuya Nakata (ORCID: https://orcid.org/0009-0001-5024-7306)
- Ayumi Gomyo (ORCID: https://orcid.org/0000-0002-1183-5412)
- Yosuke Okada (ORCID: https://orcid.org/0000-0003-4998-9078)
- Aki Tanihara
- Kazuki Yoshimura (ORCID: https://orcid.org/0000-0002-8013-1400)
- Hideki Nakasone (ORCID: https://orcid.org/0000-0001-5812-9315)
- Shinichi Kako (ORCID: https://orcid.org/0000-0002-2635-3395)
- Shun‐ichi Kimura (ORCID: https://orcid.org/0000-0001-9827-8526)
- Tomohiro Meno (ORCID: https://orcid.org/0009-0007-8790-844X)
- Nozomu Yoshino (ORCID: https://orcid.org/0000-0002-5400-6497)
- Yukiko Misaki (ORCID: https://orcid.org/0000-0002-4366-315X)
- Junko Takeshita (ORCID: https://orcid.org/0000-0003-1861-8434)
- Machiko Kusuda (ORCID: https://orcid.org/0000-0002-3313-860X)
- Shunto Kawamura (ORCID: https://orcid.org/0000-0002-7844-9233)
- Takuto Ishikawa (ORCID: https://orcid.org/0009-0002-6796-5440)
- Koki Hashino
- Kosuke Takano (ORCID: https://orcid.org/0009-0005-6052-5815)
- Kaisuke Yamamoto
Institutions
- Jichi Medical University (JP)
- Jichi Medical University Saitama Medical Center (JP)
Publication Details
- Journal
- British Journal of Haematology
- Published
- 2026-10-15
- DOI
- https://doi.org/10.1111/bjh.70881
- Primary Topic
- Hematopoietic Stem Cell Transplantation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Agency for Medical Research and Development
- Jichi Medical University
- Terumo Foundation for Life Sciences and Arts
- Japan Society for the Promotion of Science