Production of kojic acid by endophyte Neofusicoccum parvum isolated from Campomanesia adamantium and its antibacterial and anticancer application
Endophytic fungi are important sources of bioactive metabolites with applications. In this study, the ethyl acetate extract of the endophyte Neofusicoccum parvum (CA78), isolated from the leaves of Campomanesia adamantium, was characterised by NMR spectroscopy, revealing kojic acid as the predominant detectable metabolite. The extract exhibited bacteriostatic activity against Gram-negative bacteria, with minimum inhibitory concentrations of 256 μg/mL for Escherichia coli and Proteus mirabilis. In addition, the extract showed inhibitory activity against murine melanoma B16F10 cells, with an IC50 value of 42.82 µg/mL. These findings highlight the antibacterial and anticancer potential of kojic acid produced by N. parvum and reinforce the importance of endophytic fungi as sources of bioactive metabolites.
Authors
- Ana Simonet (ORCID: https://orcid.org/0000-0002-6516-1783)
- Ana Patrícia Ricci (ORCID: https://orcid.org/0000-0002-7665-1219)
- Alfred Werner Medina Loosli (ORCID: https://orcid.org/0000-0001-7288-9484)
- Júlio César Polonio (ORCID: https://orcid.org/0000-0001-5451-4320)
- Bruno Carlos Feliciano de Lima Silva (ORCID: https://orcid.org/0000-0002-2487-9801)
- Cristiano Marcelo Espínola Carvalho (ORCID: https://orcid.org/0000-0002-3867-9528)
- Ludovico Migliolo (ORCID: https://orcid.org/0000-0002-6606-2189)
- Luiz Ricardo Olchanheski (ORCID: https://orcid.org/0009-0008-9564-0241)
- Thays Barbosa da Silva
- Adriel Parahyba Lacerda (ORCID: https://orcid.org/0000-0003-4987-7922)
- Celso Vataru Nakamura
- Francielle Pelegrini Gracia
- Tiago Tognolli de Almeida
- Andressa Dalolio Valente
Institutions
- Universidade Estadual de Maringá (BR)
- Anhanguera-Uniderp University (BR)
- Universidad de Cádiz (ES)
- Universidade Católica Dom Bosco (BR)
Publication Details
- Journal
- Natural Product Research
- Published
- 2026-10-13
- DOI
- https://doi.org/10.1080/14786419.2026.2746482
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul