Influenza A virus infection induces immune dysregulation in the placenta and fetus without vertical transmission in nonhuman primates
Influenza A virus (IAV) infection during pregnancy is associated with stillbirth and preterm birth. We hypothesized that maternal IAV infection disrupts placental and fetal immune networks in ways that correlate with disease severity. Pregnant pigtail macaques were inoculated with IAV [A/California/07/2009 (H1N1)] and underwent delivery and fetal necropsy 5 days postinfection ( N = 11), with comparison to uninfected controls ( N = 16). Stillbirth occurred in 18% of infected pregnancies and in no controls. Although vertical transmission was not observed, low levels of viral RNA were detected in two placentas. Maternal infection was associated with induction of a type I interferon response in the placenta and altered innate and adaptive immune cell populations in the fetus. Maternal disease metrics (e.g., lung viral load and interferon-α) were rarely associated with placental immune perturbations but frequently linked to fetal immune cell populations (CD4 + and CD8 + T cells and monocyte subsets). Collectively, these data indicate that maternal IAV disease severity is associated with dysregulated fetal immunity.
Authors
- Raj P. Kapur (ORCID: https://orcid.org/0000-0002-5718-407X)
- Kristina M. Adams Waldorf (ORCID: https://orcid.org/0000-0002-6939-7224)
- Michelle M. Coleman (ORCID: https://orcid.org/0000-0002-6752-3266)
- Amanda Li (ORCID: https://orcid.org/0009-0008-4089-3655)
- Hazel Huang
- Hanning Li
- Mara Maughan (ORCID: https://orcid.org/0009-0003-5631-0577)
- Megan Fredericks (ORCID: https://orcid.org/0009-0002-8865-9688)
- Jake Cornelius (ORCID: https://orcid.org/0009-0003-6381-9603)
- Sidney C. Sun (ORCID: https://orcid.org/0009-0003-7254-0401)
- Christopher N. English (ORCID: https://orcid.org/0000-0002-7229-3258)
- Audrey E. Baldessari (ORCID: https://orcid.org/0000-0001-6392-3230)
- Audrey Germond
- DEBORAH HEYDENBURG FULLER (ORCID: https://orcid.org/0000-0001-7315-2441)
- Orlando Cervantes (ORCID: https://orcid.org/0000-0003-3187-9567)
- Miranda Li (ORCID: https://orcid.org/0000-0001-7541-3748)
- Thomas B. Lewis (ORCID: https://orcid.org/0009-0003-8463-6718)
- Gygeria Manuel (ORCID: https://orcid.org/0000-0002-9299-2574)
- Melissa R. Berg (ORCID: https://orcid.org/0000-0002-0053-9503)
- Hong Zhao (ORCID: https://orcid.org/0000-0003-4571-6443)
- Lakshmi Rajagopal (ORCID: https://orcid.org/0000-0003-3373-2323)
- Shannon Ruff
- Britni Curtis (ORCID: https://orcid.org/0009-0002-5824-9856)
- Edmunda Li
- Andrew E Vo (ORCID: https://orcid.org/0009-0008-2367-6691)
- Roslyn Van Abel
- Brenna Menz
- Elizabeth Miller
- Christodoulou Alexandra
- Solomon Wangari
- W. McIntyre Durning
- Jeff Munson
- Austyn Orvis
- Briana Del Rosario
Institutions
- Seattle Children's Hospital (US)
- Oregon Health & Science University (US)
- University of Washington (US)
- Seattle Children's Research Institute (US)
- Case Western Reserve University (US)
Publication Details
- Journal
- Science Advances
- Published
- 2026-10-09
- DOI
- https://doi.org/10.1126/sciadv.aee9861
- Primary Topic
- Influenza Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00