Transient receptor potential channel activation targets multiple stages of the schistosome parasite
Schistosomiasis, a neglected tropical disease caused by infection with parasitic blood flukes of the genus Schistosoma , affects more than 250 million people worldwide. Currently, patients rely on a single medicine, praziquantel (PZQ), discovered half a century ago. To combat drug resistance and reach elimination of the schistosome parasite, alternative drugs to PZQ are required that treat and prevent disease. Here, we describe a drug candidate, M5493, with potent activity against multiple parasite life cycle stages. The target is a parasite transient receptor potential ion channel, distinct from the target of PZQ. To circumvent its limited bioavailability, the active agent was converted to prodrug M5493, which displayed robust activity against the schistosome parasite at doses as low as 5 milligrams per kilogram in mice infected with Schistosoma mansoni . Unlike the benzodiazepine meclonazepam, which displays clinical antihelminthic activity through the same target, M5493 showed no sedating side effects given that it did not engage GABA A receptors. Overall, this compound has the potential to provide single-dose treatment, broad-spectrum efficacy against schistosome parasites at different life cycle stages, and transmission blocking activity.
Authors
- Evgeny G. Chulkov (ORCID: https://orcid.org/0000-0001-8030-6031)
- Nico Alexander Mell
- Christian Brenneis (ORCID: https://orcid.org/0000-0003-1814-9535)
- Claudia M. Rohr (ORCID: https://orcid.org/0000-0003-0065-7160)
- Cécile Häberli (ORCID: https://orcid.org/0000-0002-1282-8070)
- Gary Grosser (ORCID: https://orcid.org/0000-0003-2469-840X)
- Jennifer Keiser (ORCID: https://orcid.org/0000-0003-0290-3521)
- Floriane Lignet (ORCID: https://orcid.org/0000-0003-3262-6529)
- Clarissa Prazeres da Costa (ORCID: https://orcid.org/0000-0001-7996-4838)
- Jonathan S. Marchant (ORCID: https://orcid.org/0000-0001-6592-0877)
- Martin Horn (ORCID: https://orcid.org/0000-0001-9110-2018)
- Tobias Hyun Ho Baeurle (ORCID: https://orcid.org/0009-0003-0514-4126)
- Axel Becker
- Jan Dvořák (ORCID: https://orcid.org/0000-0002-6824-9000)
- Philip G. Hewitt (ORCID: https://orcid.org/0000-0002-2741-3409)
- Thomas Spangenberg (ORCID: https://orcid.org/0000-0002-5654-8919)
- Andreas D. Becker (ORCID: https://orcid.org/0000-0001-7424-2588)
- Shashank Kulkarni
- Lukas Friedrich (ORCID: https://orcid.org/0000-0001-6190-5418)
- Youssef Hamway (ORCID: https://orcid.org/0009-0002-4740-0258)
- Sang-Kyu Park (ORCID: https://orcid.org/0000-0002-3875-1227)
- Rana Afshar
- Annika Hansen
- Mario Pellegatti (ORCID: https://orcid.org/0000-0002-4866-336X)
- Jesko Kaiser
Institutions
- University of Verona (IT)
- Swiss Tropical and Public Health Institute (CH)
- Merck KGaA, Darmstadt (Germany) (DE)
- University of Basel (CH)
- Czech Academy of Sciences (CZ)
- Medical College of Wisconsin (US)
- Czech University of Life Sciences Prague (CZ)
- German Center for Infection Research (DE)
- Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry (CZ)
- Merck Healthcare KGaA, Darmstadt (Germany) (DE)
- Technical University of Munich (DE)
Publication Details
- Journal
- Science Translational Medicine
- Published
- 2026-10-07
- DOI
- https://doi.org/10.1126/scitranslmed.aed8353
- Primary Topic
- Parasites and Host Interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00