Enzyme-Responsive Chemiluminescent Nanofibrous Membranes for Monitoring and Photothermal Therapy of Infected Wounds

Abstract Rapid detection and effective elimination of wound infection are imperative for wound management. Herein, we designed a chemiluminescence nanofiber membrane (CL-PVA/PLA@PB NCs) that integrates visual diagnosis and on-site photothermal disinfection for enhancing wound healing. The composited nanofiber membrane was fabricated from adamantyl-phenoxy-1,2-dioxetane-modified polyvinyl alcohol (CL-PVA) and polylactic acid (PLA) and loaded with a photothermal agent, Prussian blue nanocubes (PB NCs). Upon interaction with alkaline phosphatase secreted (ALP) by bacteria, strong chemiluminescence from CL-PVA/PLA@PB NCs was observed, allowing us to detect bacteria at the site of infected wounds and evaluate the wound infection status. Furthermore, under near-infrared (NIR) laser irradiation, the excellent photothermal effect induced by PB NCs endowed the membrane with potent antibacterial properties. Meanwhile, in vivo experiments showed that the CL-PVA/PLA@PB NCs with excellent biocompatibility could achieve chemiluminescence-guided photothermal antibacterial therapy, accelerating wound healing. Chemiluminescence-guided photothermal antibacterial therapy based on CL-PVA/PLA@PB NCs provided a new approach for wound monitoring and therapy.