Agro-waste mediated detoxification of textile effluent coupled with laccase biosynthesis and its application in mung bean cultivation

This study demonstrates the use of a combination of physical and biological methods for the treatment of textile effluent using sugarcane bagasse as an adsorbent. Sugarcane bagasse adsorbed with textile effluent was used to produce laccase from a thermophilic bacterial strain. The treated effluent was utilized for the cultivation of mung beans. After the physical treatment, the effluent had a biological oxygen demand of 35 mg L -1 with 3.9 g L -1 total dissolved solids and heavy metals in the permissible range. The data showed that physical growth parameters (number of leaves, leaf area, weight and length of shoot, root and leaves) and nutritional profile (protein, glucose, antioxidant, phenol and flavonoid content) of mung bean plants grown by irrigation with treated effluent were improved. Furthermore, effluent-adsorbed matrix was fermented by a thermophile, Neobacillus sedimentimangrovi UE25 which yielded 50 IU mL -1 laccase and 66.30 IU mL -1 endoglucanase. Remarkably, this is the first report of laccase production by any species of the genus Neobacillus , highlighting the novel enzymatic potential this strain. This research paves the way to provide a solution for the treatment of textile effluent with the subsequent production of commercially important enzymes.