Coadministration of fluoride, trimetaphosphate, and quercetin reduces dentin erosive wear in vitro

Abstract Objectives To evaluate the effect of solutions containing quercetin (QC), sodium trimetaphosphate (TMP), and fluoride (F), in different combinations, on dentin erosive wear (DEW) in vitro. Materials and methods Bovine root dentin blocks were randomly divided into seven experimental groups ( n = 28/group): deionized water (control); 500 ppm F (500F); 1100 ppm F (1100F); 5000 ppm F (5000F); 1100 F + 0.03% QC (F + QC); 1100 F + 1% TMP (F + TMP); and 1100 F + 1% TMP + 0.03% QC (“EXP” - experimental). Blocks were treated twice/day and subjected to four daily erosive challenges for five days (0.05 M citric acid, pH 3.2, 90 s), either erosion only (ERO) or erosion plus abrasion by brushing (ERO + ABR). The samples were analyzed by profilometry and computed X-ray microtomography, and the data were analyzed by 2-way ANOVA followed by Fisher’s LSD post hoc test. Results Under both conditions, a dose-response relationship was observed between F concentration and all variables, with the lowest values for 5000F and EXP. Under ERO, only 5000F showed lower DEW than 1100F; under ERO + ABR, all TMP and/or QC-containing solutions reduced DEW compared with 1100F, with a similar trend for mineral loss and depth of mineral loss, most notably for 5000F and EXP. Conclusions Adding QC or TMP to 1100F produced modest additional protection, while their combination yielded a significant additive effect comparable to that of a solution with a five-fold higher F concentration. Clinical relevance Possibility of reducing fluoride concentration in preventive formulations with an effect similar to that of high-concentration formulations against DEW.