Spectrum of Gram-Positive Cocci and Their Antimicrobial Susceptibility in Delayed Surgical Site Infections in Mymensingh, Bangladesh.

Surgical site infection is one of the most frequent post-operative consequences are infections caused by invasive surgical procedures that occur within 30 days if the patient does not have a prosthetic in place or within a year if a prosthetic is implanted in the patient. Many Gram-positive and Gram-negative bacteria are causing surgical site infections. The objective of this study was to isolate and identify the Gram-Positive Cocci and their antibiotic susceptibility patterns from patients with delayed surgical site infections in Mymensingh Medical College Hospital of Bangladesh. This cross-sectional study involved 155 patients suffering from postsurgical wound infection more than three weeks as respondents. The study was conducted in the Department of Microbiology, Mymensingh Medical College, Bangladesh from March 2024 to February 2025. The bacterial pathogens were isolated from pus/ wound swab samples obtained from those patients. The Gram-Positive Cocci were identified through several standard biochemical tests and finally, the culture sensitivity tests of those bacterial isolates were performed by disk diffusion method. Data was documented using standard questionnaires. The mean age of the patients in the study was 33.97 years. Most of the patients 96(61.9%) were female. Out of a total 155 samples, 143(92.30%) were positive by culture. Among them Gram positive were 16(11.2%); Staphylococcus aureus was 11(68.75%), coagulase negative Staphylococcus (CONS) was 4(25.0%) and Enterococcus spp. was 1(6.25%). Among the isolated Staphylococcus aureus, 6(54.54%) were found MRSA. The Staphylococcus aureus showed 100.0% sensitivity to meropenem and linezolid and 100.0% resistant to Ceftriaxone. The Coagulase negative Staphylococcus isolates were 100.0% sensitive to cefoxitin and showed resistant to most the drugs. The Enterococcus spp. was sensitive to amoxiclav, linezolid and Pipercillin-Tazobactum. Meropenem and linezolid have shown better activity on to S. aureus. CoNS exhibited higher resistance to many antibiotics compared to S. aureus.