Concentration-Dependent Inhibitory Effect Of Calcium Fructoborate On Sirt1 Enzyme Activity: A Comparative Analysis With Boric Acid

Purpose: SIRT1 functions in regulatory mechanisms including cellular aging, apoptosis, glucose and lipid metabolism, oxidative stress and inflammation. Studies show that SIRT1 inhibitors may affect various types of cancer and metabolic disorders, making SIRT1 an important therapeutic target. Boric acid is an inorganic boron compound with antimicrobial, antifungal and potential anticancer effects. Calcium fructoborate, another boron compound found in vegetables and fruits, has anti-inflammatory, antioxidant and anti-tumor effects. This study aimed to investigate the inhibitory potential of calcium fructoborate on SIRT1 activity and to evaluate its possible use as a chemical tool for future studies targeting SIRT1 regulation.Material and Methods: In this study, the effect of calcium fructoborate on SIRT1 activity was investigated by a fluorescence-based method using human recombinant SIRT1.Results: Calcium fructoborate inhibited SIRT1 activity in a dose-dependent manner. Calcium fructoborate significantly inhibited SIRT1 activity by 42% at a concentration of 20 mM (p < 0.001). In the boric acid group, a decreasing trend in SIRT1 enzyme activity was observed, but this difference was not statistically significant.Conclusion: The results provide important basic information. Calcium fructoborate may be used as a chemical tool to elucidate the biological functions of SIRT1 and to investigate the potential therapeutic applications of SIRT1 inhibitors.