Dataset and analysis scripts for: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM Study

This repository contains the raw data and analysis scripts supporting the manuscript below. Manuscript: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM StudyTarget journal: Nanoscale (RSC)Corresponding author / data contact: [email protected] It includes raw AFM-QI data (.gwy), correlative fluorescence microscopy images, ROI sets used for spot localization, NTA export files, and the Python scripts used for data processing, colocalization analysis, and figure generation. Folder structure and file naming are described in the accompanying README. Raw data files are licensed under CC-BY 4.0; analysis scripts (.py) are licensed under the MIT License.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2026-09-30
DOI
https://doi.org/10.5281/zenodo.23041450
Primary Topic
Extracellular vesicles in disease
Type
article
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Dataset and analysis scripts for: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM Study

Jaroslaw Jacak, Fabian Hauser, Wolfgang Holnthoner, Dmitry Sivun et al.
Zenodo (CERN European Organization for Nuclear Research)
Extracellular vesicles in disease
article

Dataset and analysis scripts for: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM Study

Jaroslaw Jacak, Fabian Hauser, Wolfgang Holnthoner, Dmitry Sivun, Markus Axmann, Johannes Grillari, Madhusudhan Reddy Bobbili, Sascha Raschke, Barbara Kroenigsberger, Najmeddin Dery
article en

Abstract

This repository contains the raw data and analysis scripts supporting the manuscript below. Manuscript: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM StudyTarget journal: Nanoscale (RSC)Corresponding author / data contact: [email protected] It includes raw AFM-QI data (.gwy), correlative fluorescence microscopy images, ROI sets used for spot localization, NTA export files, and the Python scripts used for data processing, colocalization analysis, and figure generation. Folder structure and file naming are described in the accompanying README. Raw data files are licensed under CC-BY 4.0; analysis scripts (.py) are licensed under the MIT License.

Zenodo (CERN European Organization for Nuclear Research)
University of Vienna (AT), Ludwig Boltzmann Gesellschaft (AT), Max Perutz Labs (AT), Upper Austrian Research (AT), Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA (AT), University of Applied Sciences Upper Austria (AT), Vienna Biocenter (AT), BOKU University (AT)
Openalex Percentile: Top 19%
Extracellular vesicles in disease
DOIOpen Access PDFOpenAlex
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Dataset and analysis scripts for: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM Study — Jaroslaw Jacak, Fabian Hauser, et al. · Zenodo (CERN European Organization for Nuclear Research) (2026) | TGRS Research Map | TGRS