PS1-21. Organic Minerals Modulate Hepatic Mitochondrial Metabolism and Inflamatory Signaling in Feedlot Cattle.

Abstract Trace mineral source can influence metabolic processes related to growth, health, and productivity in feedlot cattle. As part of a broader research project investigating trace mineral supplementation strategies in beef cattle, this study aimed to evaluate hepatic proteomic responses to carbo-amino-phospho-chelated trace minerals compared with conventional sulfate-based mineral sources during the finishing period. Sixteen crossbred Nellore × Angus bulls (initial BW = 472 ± 4.9 kg; 18mo of age) were fed a high-concentrate diet for 89 d and randomly assigned to one of two mineral sources: (1) INORG, with inorganic sulphate-based mineral sources, or (2) TM, with carbo-amino-phospho-chelated trace minerals (Cr, Zn, Mn, Se, S, Cu, and Co; Tortuga® Minerals, DSM-Firmenich, São Paulo, Brazil). At the end of the finishing period, cattle were slaughtered and liver samples were collected immediately after slaughter and stored at − 80 °C until analysis. Proteomic profiling was conducted using a label-free quantitative approach on a high-resolution ACQUITY I-Class Xevo G2-XS mass spectrometry system (Waters, Manchester, UK), and protein identification and quantification were performed using Progenesis QI for Proteomics software. Proteomic analysis revealed distinct hepatic responses between mineral sources. A total of 3,571 proteins were identified, of which 38 showed differential abundance (DA; P < 0.05). Animals receiving TM showed greater abundance of NADH-ubiquinone oxidoreductase (P = 0.0378), an enzyme involved in mitochondrial oxidative phosphorylation and ATP production, suggesting enhanced cellular bioenergetic efficiency. In contrast, sulphate-based mineral supplementation increased the abundance of several proteins associated with metabolic remodeling and inflammatory signaling, including UQCRC2 (P = 0.0345), ASGR1 (P = 0.0271), ABHD10 (P = 0.0020), RABEP1 (P = 0.0328), NACA (P = 0.0378), and LMNA (P = 0.0029). Additionally, proteins frequently associated with inflammatory and cellular stress responses, such as TLN1, SHMT, SUSD, UCHs, MRP4, CUEDC1, SECISBP2, AGO1, RTK, P115, KRT8, and HECT-type E3 ubiquitin transferases, were more abundant in animals receiving INORG (P < 0.05). Abundance of ANKRD26 (P = 0.0320), a regulator of the ERK/mTOR signaling pathway involved in protein synthesis and nutrient metabolism, was also greater in animals receiving sulphaate-based minerals. These findings suggest that trace mineral source modulates hepatic mitochondrial metabolism and inflammatory signaling in feedlot cattle, indicating that carbo-amino-phospho-chelated minerals supplementation may enhance cellular bioenergetic efficiency, whereas sulphate-based minerals were associated with greater abundance of proteins related to inflammatory signaling and metabolic stress responses, providing mechanistic insight into hepatic metabolic regulation.