Intracranial administration of surface modified PAMAM dendrimer migrates to the infarct area in the MCAo rat model of stroke.

Ischemic stroke induces hypoxia-driven neuroinflammation, leading to neuronal death and glial scar formation that hinders recovery. In this study, we evaluated Cy5.5-tagged generation 4 PAMAM dendrimer as a potential therapeutic delivery vector in a middle cerebral artery occlusion (MCAo) rat model. Primary astrocyte cultures confirmed GFAP expression and dendrimer uptake. Intracranial injections and in-vivo brain imaging revealed Cy5.5 localization in the brain. Immunohistochemistry revealed Cy5.5 localization near the infarct area as well as in astrocytes, neurons, and microglia. We did not observe any significant reduction in infarct volume nor observed behavioral alteration. These findings establish G4 90/10-Cy5.5 as an inert, a safe, biocompatible nanocarrier for targeted post-stroke therapeutic delivery.

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Publication Details

Journal
Next Nanotechnology
Published
2026-09-29
DOI
https://doi.org/10.1016/j.nxnano.2026.100817
Primary Topic
Dendrimers and Hyperbranched Polymers
Type
article
Field-Weighted Citation Impact
0.00

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article

Intracranial administration of surface modified PAMAM dendrimer migrates to the infarct area in the MCAo rat model of stroke.

Melissa Andrews, Gary Leo Dunbar, Harrison Schurr, Julien Rossignol et al.
Next Nanotechnology
Dendrimers and Hyperbranched Polymers
article

Intracranial administration of surface modified PAMAM dendrimer migrates to the infarct area in the MCAo rat model of stroke.

Melissa Andrews, Gary Leo Dunbar, Harrison Schurr, Julien Rossignol, Balachandar Kathirvelu, Bhairavi Srinageshwar, Ajit Sharma, Douglas R. Swanson, Clayton E. Malkowski, Bethany MacDonald, Justin Stadler, Grant Raymor, Arjun Poudel, Melissa R. Resk, Sarah Peruzzaro, Joseph Hellrung, Alexa K. Toth
article en

Abstract

Ischemic stroke induces hypoxia-driven neuroinflammation, leading to neuronal death and glial scar formation that hinders recovery. In this study, we evaluated Cy5.5-tagged generation 4 PAMAM dendrimer as a potential therapeutic delivery vector in a middle cerebral artery occlusion (MCAo) rat model. Primary astrocyte cultures confirmed GFAP expression and dendrimer uptake. Intracranial injections and in-vivo brain imaging revealed Cy5.5 localization in the brain. Immunohistochemistry revealed Cy5.5 localization near the infarct area as well as in astrocytes, neurons, and microglia. We did not observe any significant reduction in infarct volume nor observed behavioral alteration. These findings establish G4 90/10-Cy5.5 as an inert, a safe, biocompatible nanocarrier for targeted post-stroke therapeutic delivery.

Next NanotechnologyVol. 10
Central Michigan University (US)
American Heart Association
Openalex Percentile: Top 25%
Dendrimers and Hyperbranched Polymers
DOIOpen Access PDFOpenAlex
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Intracranial administration of surface modified PAMAM dendrimer migrates to the infarct area in the MCAo rat model of stroke. — Melissa Andrews, Gary Leo Dunbar, et al. · Next Nanotechnology (2026) | TGRS Research Map | TGRS