PS13-4. Effect of Adding Plant Extracted Hydrolysable Tannin and Saponin Combination in Blend Pelleted Products on Protein Degradable and Undegradable Fraction Profiles and Their Total Supply.

Abstract The objectives of this study were to determine effect of adding plant extracted hydrolysable tannin (HT) and saponin (S) combination as a combined phytochemical feed additive (HTS) on Protein Degradable and Undegradable Fraction Profiles and Total Supply of blend pelleted products for dairy cows. The plant extracted HT and S were combined with four different levels, which included: Level 1 = 0 (control, 0 %HT+0 %S of diet DM), level 2 = 1.5 (1%HT+0.5%S of diet DM ), level 3 = 3 (2%HT+1%S of diet DM), and Level 4 = 4.5 (3%HT+1.5%S of diet DM)]. Then these combined additives (HTS) were added to canola meal and pea mixtures with canola meal to pea mixture ratio of 50:50. Total eight blend pelleted feed products (= 4 additive levels x 2 ratios of canola meal and pea mixtures =8) were produced at Canadian Feed Research Center (CFRC, University of Saskatchewan, Canada). Blend pelleted feed products were produced at Canadian Feed Research Center (CFRC, University of Saskatchewan, Canada). The protein degradable and undegradable fraction profiles and their total supply were determined using the updated Cornell Net Carbohydrate and Protein System. The experimental design was a CRD with the combined phytochemical feed additive (HTS) level as a fixed effect. The data was analyzed using Mixed Model Procedure of SAS. Orthogonal polynomial contracts were used to determine linear and quadratic relationship between HTS dosage level and various protein degradable and undegradable fractions and total supply. The results showed that with increasing HTS level from 0 to 4.5%, insoluble protein fraction (PB1) was quadratically increased (P = 0.041) from 14.1 to 16.2 % DM, therefore rumen degradable (RDPB1) and undegradable protein PB1 fractions (RUPB1) were also quadratically increased (P = 0.041) from 6.9 to 7.9 % DM and 7.2 to 8.3 %DM, respectively. However, the dosage of HTS did not have significant impact on the fiber-bound protein (PB2), the soluble crude protein (PA2) and indigestible protein fraction (PC). Although, adding HTS to Prairie blend pellet feed products significantly affected protein subfractions (PB1, RDPB1, RUPB1), it did not significantly impact total degradable (TRDP) and undegradable protein supply (TRUP) to ruminants with average of 20.7%DM and 11.8% DM, respectively