Microbiological and chemical safety of artisanal dark and white chocolate bars from small-scale industries in West Java, Indonesia

Global chocolate production has grown steadily, and Indonesia, the world's third-largest cacao producer, has seen a parallel rise in artisanal chocolate manufacturing in West Java. Food safety data for these small-scale producers remain limited. West Java's volcanic soils are rich in geogenic cadmium, which Theobroma cacao absorbs and concentrates in the bean regardless of processing, and the rapid growth of micro-enterprises without validated food-safety systems raises concerns for both microbiological hygiene and heavy-metal compliance. Sixteen chocolate bars (eight dark, eight white) from eight small-scale West Java producers were analysed. Total viable count (TVC), total yeast and mould count (TYMC), and Salmonella spp. were evaluated per ISO standards; lead (Pb) and cadmium (Cd) were measured by ICP-MS. A linear mixed-effects model treated chocolate type as a fixed effect and brand as random. Compliance was checked against Indonesian (BPOM), EU, FSANZ, and Codex standards. All samples met BPOM's microbiological limits, with TVC of 2.5 × 103–4.0 × 103 CFU/g, TYMC of 1.2 × 102–8.5 × 102 CFU/g, and no Salmonella spp. detected. Chocolate type significantly affected TYMC (dark > white; p = 0.003) but not TVC ( p = 0.12); brand variation was significant for both ( p < 0.001). All samples met BPOM's Cd limits, but one brand's white chocolate (0.105 ± 0.015 mg/kg Cd) slightly exceeded the EU limit of 0.10 mg/kg. Cocoa-solid content correlated strongly with Cd ( r = 0.92, p < 0.05), an association consistent with, though not direct evidence of, the region's volcanic-soil geochemistry. West Java's artisanal chocolate producers show satisfactory microbiological safety and effective lead management, but cadmium governance needs priority, particularly for European markets. Given the purposive sampling and limited number of brands, findings are indicative rather than sector-wide. Future work should expand sampling, map soil cadmium variability, and quantify dietary cadmium exposure among Indonesian consumers.

Authors

Institutions

Publication Details

Journal
Food Safety and Risk
Published
2026-09-26
DOI
https://doi.org/10.1186/s40550-026-00166-w
Primary Topic
Heavy metals in environment
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
article

Microbiological and chemical safety of artisanal dark and white chocolate bars from small-scale industries in West Java, Indonesia

Hanifah Nuryani Lioe, Gisubizo Fabien, Harsi Dewantari Kusumaningrum, Uswatun Hasanah
Food Safety and Risk
Heavy metals in environment
article

Microbiological and chemical safety of artisanal dark and white chocolate bars from small-scale industries in West Java, Indonesia

Hanifah Nuryani Lioe, Gisubizo Fabien, Harsi Dewantari Kusumaningrum, Uswatun Hasanah
article en

Abstract

Global chocolate production has grown steadily, and Indonesia, the world's third-largest cacao producer, has seen a parallel rise in artisanal chocolate manufacturing in West Java. Food safety data for these small-scale producers remain limited. West Java's volcanic soils are rich in geogenic cadmium, which Theobroma cacao absorbs and concentrates in the bean regardless of processing, and the rapid growth of micro-enterprises without validated food-safety systems raises concerns for both microbiological hygiene and heavy-metal compliance. Sixteen chocolate bars (eight dark, eight white) from eight small-scale West Java producers were analysed. Total viable count (TVC), total yeast and mould count (TYMC), and Salmonella spp. were evaluated per ISO standards; lead (Pb) and cadmium (Cd) were measured by ICP-MS. A linear mixed-effects model treated chocolate type as a fixed effect and brand as random. Compliance was checked against Indonesian (BPOM), EU, FSANZ, and Codex standards. All samples met BPOM's microbiological limits, with TVC of 2.5 × 103–4.0 × 103 CFU/g, TYMC of 1.2 × 102–8.5 × 102 CFU/g, and no Salmonella spp. detected. Chocolate type significantly affected TYMC (dark > white; p = 0.003) but not TVC ( p = 0.12); brand variation was significant for both ( p < 0.001). All samples met BPOM's Cd limits, but one brand's white chocolate (0.105 ± 0.015 mg/kg Cd) slightly exceeded the EU limit of 0.10 mg/kg. Cocoa-solid content correlated strongly with Cd ( r = 0.92, p < 0.05), an association consistent with, though not direct evidence of, the region's volcanic-soil geochemistry. West Java's artisanal chocolate producers show satisfactory microbiological safety and effective lead management, but cadmium governance needs priority, particularly for European markets. Given the purposive sampling and limited number of brands, findings are indicative rather than sector-wide. Future work should expand sampling, map soil cadmium variability, and quantify dietary cadmium exposure among Indonesian consumers.

Food Safety and RiskVol. 13(1)
IPB University (ID), University of Rwanda (RW)
Openalex Percentile: Top 22%
Heavy metals in environment
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.