Recurrent Transcriptomic Organization Across Sample Groups in Pediatric Neuroblastoma: A Prospectively Constrained Single-Cell Analysis

Neuroblastoma exhibits substantial transcriptional heterogeneity and developmental-state organization. This study evaluated whether a recurrent transcriptomic pattern could be identified across source-defined Tumour cluster 1 (TC1) groups in an independently derived pediatric neuroblastoma single-cell dataset under prospectively specified analytical constraints. Three TC1 SampleName groups met the prespecified quantitative eligibility criterion. Recurrent expression required concordant median-expression direction across all three eligible TC1 comparisons against within-sample Leukocytes. A total of 569 genes met this criterion, comprising 505 recurrent higher-expression and 64 recurrent lower-expression genes. Functional enrichment of the complete recurrent gene sets identified organized representation across mitochondrial-energy/respiration, metabolism, cell-cycle/proliferation, translation and protein processing, neural/neurodevelopmental, cytoskeletal, immune/antigen-presentation, and additional functional categories. The findings demonstrate reproducible-within-dataset TC1 transcriptomic organization across three eligible SampleName groups but do not establish patient-level replication, causal mechanism, clinical relevance, or tissue-level architectural control. The available measurements did not directly evaluate the frozen BOCM relationships or candidate convergence points; therefore, BOCM-H01 remains NOT_EVALUABLE_FROM_GOSH_ALONE. The study is presented as a bounded independent pediatric transcriptomic challenge and characterization rather than direct validation or falsification of BOCM.