RootQuant held-out test set: maize and soybean minirhizotron images with manually traced root length and root surface area

A lack of high-throughput phenotyping solutions for root traits in field-grown crops has severely constrained understanding and improvement of below-ground traits and processes. Minirhizotrons are the standard non-destructive root-phenotyping method in field environments. Computer vision solutions are needed to allow automated trait estimation at scale, but training data is scarce and human annotations are often inaccessible because they reside in proprietary software that only exports per-image scalar totals of root length and surface area. Nevertheless, large numeric archives of these root traits already exist. RootQuant showed that the traits can be predicted directly from the whole image by regression, thus removing manually traced masks from the pipeline; RootQuantV2 takes that idea further by replacing RootQuant's CNN backbone with a self-supervised ViT. We adapt a frozen DINOv3 ViT-L/16 with a hybrid parameter-efficient scheme. Training only 11.9M parameters (3.78% of the model), RootQuantV2 achieves length and area $R^2$ of 0.950 and 0.930, respectively, while lowering length/area RMSE by 24.3%/20.7% over RootQuant. RootQuantV2 thus repurposes legacy numeric archives for high-throughput, automated root trait estimation.

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Publication Details

Journal
PubMed
Published
2026-10-06
DOI
https://doi.org/10.5281/zenodo.23174035
Primary Topic
Smart Agriculture and AI
Type
preprint

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preprint

RootQuant held-out test set: maize and soybean minirhizotron images with manually traced root length and root surface area

Andrew D. B. Leakey, Dylan Allen, Jeremy Ruhter, Kinjalk Parth et al.
PubMed
Smart Agriculture and AI
preprint

RootQuant held-out test set: maize and soybean minirhizotron images with manually traced root length and root surface area

Andrew D. B. Leakey, Dylan Allen, Jeremy Ruhter, Kinjalk Parth, Samuel Walker, Sebastian Varela, Ashish Rajurkar, Scott McCoy
preprint en

Abstract

A lack of high-throughput phenotyping solutions for root traits in field-grown crops has severely constrained understanding and improvement of below-ground traits and processes. Minirhizotrons are the standard non-destructive root-phenotyping method in field environments. Computer vision solutions are needed to allow automated trait estimation at scale, but training data is scarce and human annotations are often inaccessible because they reside in proprietary software that only exports per-image scalar totals of root length and surface area. Nevertheless, large numeric archives of these root traits already exist. RootQuant showed that the traits can be predicted directly from the whole image by regression, thus removing manually traced masks from the pipeline; RootQuantV2 takes that idea further by replacing RootQuant's CNN backbone with a self-supervised ViT. We adapt a frozen DINOv3 ViT-L/16 with a hybrid parameter-efficient scheme. Training only 11.9M parameters (3.78% of the model), RootQuantV2 achieves length and area $R^2$ of 0.950 and 0.930, respectively, while lowering length/area RMSE by 24.3%/20.7% over RootQuant. RootQuantV2 thus repurposes legacy numeric archives for high-throughput, automated root trait estimation.

PubMed
University of Illinois Urbana-Champaign (US), Digital Science (United States) (US), Center for Advanced Bioenergy and Bioproducts Innovation (US), Carl R. Woese Institute for Genomic Biology (US)
National Science Foundation, U.S. Department of Energy, U.S. Department of Agriculture, Advanced Research Projects Agency - Energy, National Institute of Food and Agriculture, Office of Science, Advanced Research Projects Agency, Biological and Environmental Research
Zero hunger
Smart Agriculture and AI
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