ABCC1, NCF4 and CBR3 polymorphisms in Jordanian acute lymphoblastic leukaemia: insights into laboratory and clinical correlates

Acute lymphoblastic leukemia is a blood cancer characterized by uncontrolled proliferation of immature lymphoid cells. Genetic variations such as ABCC1 rs3743527, NCF4 rs1883112 and CBR3 rs1056892 may influence disease progression and consequently the response to treatment. The purpose of this study was to determine the frequency of ABCC1 rs3743527, NCF4 rs1883112 and CBR3 rs1056892 variants in Jordanian patients with ALL and to investigate their potential associations with laboratory findings and disease characteristics. This case–control study included 52 ALL patients and 35 healthy controls. Clinical and laboratory data were collected from hospital medical records. Genomic DNA was extracted from peripheral blood, and genotyping was performed using rhAmp™ SNP Assays based on real-time PCR. All SNPs were in Hardy–Weinberg equilibrium in controls. The most common genotypes were CC (ABCC1), GA (NCF4) and GA (CBR3). No significant association was found between these variants and ALL risk. Significant genotype‑related differences were observed for Hb (adjusted p = 0.027) and Hct (adjusted p = 0.005) and MCH (adjusted p = 0.016) in CBR3 rs1056892. Collectively, these variants were not associated with ALL risk in Jordanian patients. Notably, this is the first study in Jordan to investigate these SNPs in ALL patients.