Standardization of Protodioscin Quantification in Tribulus Terrestris Linn to Resolve Analytical Variances

Tribulus terrestris is a highly potent medicinal plant belonging to the caltrop family. This study reviews its wide range of therapeutic applications and health benefits. The plant exhibits strong diuretic and anti-urolithiatic properties, making it effective for promoting urination and preventing kidney stones. Additionally, it serves as an antimicrobial and anthelminthic agent to combat infections and parasites. Cardiotonic and hepatoprotective actions ensure the protection of vital organs like the heart and liver. It effectively manages metabolic health through Hypolipidemic and antidiabetic activities that lower cholesterol and blood sugar levels. Furthermore, the plant offers pain relief, reduces inflammation, and prevents muscle spasms due to its analgesic, anti-inflammatory, and antispasmodic traits. As an aphrodisiac and immunomodulator, it enhances sexual health and strengthens the immune system. Finally, its antioxidant, anti-tumor, and anti-cariogenic properties help neutralize cellular damage, fight cancer cells, and protect dental health, highlighting its vast pharmaceutical potential The active compound, protodioscin, was identified and measured in the fruit extract of Tribulus terrestris using advanced testing methods. First, a UV spectrophotometer confirmed the compound by showing its peak light absorption at 205 nm. Then, TLC and HPLC testing methods were used to find the exact amount of protodioscin present in the fruit. Protodioscin is the substances of potential therapeutic interest are steroidal saponins. The following furostanol saponins-protodioscin, neoprotodioscin and their respective sulphates methylprotodioscin, prototribestrin, methylprorotribestrin and many more. Overall review of protodioscin medicinal applications like kidney and urinary tract health, sexual and reproductive wellness, athletic performance and muscle building and general health and wellness.