Self‐Supervised Internal Learning Enhances Isotropic Resolution for Three‐Dimensional Fluorescence Microscopy

ABSTRACT Three‐dimensional fluorescence microscopy often exhibits anisotropic resolution because axial information is poorly sampled and more blurred than lateral information, which complicates quantitative interpretation of fine 3D structures. Although optical remedies and computational restoration have been explored, many approaches require demanding system calibration or rely on accurate PSF models and assumptions that are difficult to satisfy across all samples and modalities. Here we present DeepIso, a self‐supervised isotropy restoration framework that couples supervised pretraining with an internal‐learning inference stage to estimate degradation directly from the measured volume. Without explicit PSF specification or enforced lateral–axial structural equivalence, DeepIso recovers axial frequency content and improves the continuity of elongated structures while retaining fine features, with superior performance over existing computational approaches in terms of both visual inspection and quantitative metrics. The method is validated on synthetic benchmarks and experimental datasets, demonstrating isotropy enhancement across confocal, light‐sheet, and 3D structured illumination microscopy, thereby supporting downstream volumetric analysis including segmentation and tracking.