Deamidated Rice Protein as a Functional Fortificant in Wheat Flatbread

ABSTRACT Background and Objectives Wheat flatbread is a major dietary staple but has limited protein quality. This study evaluated native rice protein isolate (RPI) and rice protein isolate deamidated using a natural deep eutectic solvent (NADES) system (dRPI; 1%–5%, w/w) as clean‐label fortificants to improve the nutritional properties of whole‐wheat flatbread. Findings Fortification enhanced protein quality, increasing lysine from 0.270 g/100 g in the control to 0.368 g/100 g in flatbread fortified with 5% dRPI. Protein Digestibility‐Corrected Amino Acid Score (PDCAAS) increased from 41.8% to 47.1%, while the predicted glycemic index decreased by up to 16.8%. Fortified doughs remained processable, with arrival and departure times comparable to the control, moderate increases in dough development time (4.4 to 4.5–6.0 min), and dough stability remaining within a workable range across treatments (3.1–4.3 min). Although both variants showed acceptable sensory characteristics, dRPI‐fortified flatbreads achieved better acceptance than RPI‐fortified samples, which showed greater grittiness at higher inclusion levels. Conclusion Both fortificants improved nutritional quality, but dRPI maintained better functional and sensory performance, with 3% dRPI offering the best overall balance. Significance and Novelty NADES‐deamidated rice protein is a promising clean‐label fortificant for nutritionally improved wheat flatbread.

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Publication Details

Journal
Cereal Chemistry
Published
2026-09-22
DOI
https://doi.org/10.1002/cche.70112
Primary Topic
Food composition and properties
Type
article
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article

Deamidated Rice Protein as a Functional Fortificant in Wheat Flatbread

Asifa Shahid, Muhammad Abdul Haq, Naveed Hussain
Cereal Chemistry
Food composition and properties
article

Deamidated Rice Protein as a Functional Fortificant in Wheat Flatbread

Asifa Shahid, Muhammad Abdul Haq, Naveed Hussain
article en

Abstract

ABSTRACT Background and Objectives Wheat flatbread is a major dietary staple but has limited protein quality. This study evaluated native rice protein isolate (RPI) and rice protein isolate deamidated using a natural deep eutectic solvent (NADES) system (dRPI; 1%–5%, w/w) as clean‐label fortificants to improve the nutritional properties of whole‐wheat flatbread. Findings Fortification enhanced protein quality, increasing lysine from 0.270 g/100 g in the control to 0.368 g/100 g in flatbread fortified with 5% dRPI. Protein Digestibility‐Corrected Amino Acid Score (PDCAAS) increased from 41.8% to 47.1%, while the predicted glycemic index decreased by up to 16.8%. Fortified doughs remained processable, with arrival and departure times comparable to the control, moderate increases in dough development time (4.4 to 4.5–6.0 min), and dough stability remaining within a workable range across treatments (3.1–4.3 min). Although both variants showed acceptable sensory characteristics, dRPI‐fortified flatbreads achieved better acceptance than RPI‐fortified samples, which showed greater grittiness at higher inclusion levels. Conclusion Both fortificants improved nutritional quality, but dRPI maintained better functional and sensory performance, with 3% dRPI offering the best overall balance. Significance and Novelty NADES‐deamidated rice protein is a promising clean‐label fortificant for nutritionally improved wheat flatbread.

Cereal Chemistry
Hamdard University (PK), University of Karachi (PK), Ziauddin University (PK)
Zero hunger
Openalex Percentile: Top 12%
Food composition and properties
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Deamidated Rice Protein as a Functional Fortificant in Wheat Flatbread — Asifa Shahid, Muhammad Abdul Haq, et al. · Cereal Chemistry (2026) | TGRS Research Map | TGRS