Longitudinal Analysis of Matched Patient Biospecimens Reveals Neural Reprogramming of Cancer-Associated Fibroblasts Following Chemotherapy in Pancreatic Cancer
Pancreatic ductal adenocarcinoma (PDAC), the most common subtype of pancreatic cancer, is a deadly disease with a complex tumor microenvironment (TME). Cytotoxic combination chemotherapy treatments are the mainstay of PDAC therapy, but patients rapidly develop chemoresistance, highlighting the need to elucidate how chemotherapy alters the TME and whether these changes drive chemoresistance. Here, we examined matched pre- and post-treatment tissue specimens using single-cell RNA-sequencing and found near-universal enrichment after treatment of axonal guidance genes in cancer-associated fibroblasts (CAFs). These neural CAFs were enriched near sites of perineural invasion, coinciding with regions of increased tumor cell proliferation, and were enriched in tumor areas distant from nerves after chemotherapy. Metastatic recurrent lesions had the highest prevalence of neural CAFs versus primary tumors and untreated metastases. Neural CAFs showed elevated non-canonical WNT mediators and axonal guidance genes, which complemented matching cognate binding partners in tumor epithelial cells, suggesting a role in tumor-stroma crosstalk. Additionally, in vitro studies revealed enrichment of axonal guidance genes in patient-derived CAF lines upon exposure to chemotherapy or tumor cells. Altogether, these findings implicate fibroblast-derived axonal-guidance genes in promoting PDAC invasion and point to a promising therapeutic target for this disease.
Authors
- Richard S. Kwon (ORCID: https://orcid.org/0000-0002-4652-1106)
- Ahmed M. Elhossiny (ORCID: https://orcid.org/0000-0003-0884-8754)
- George Philips (ORCID: https://orcid.org/0009-0008-7304-721X)
- Eileen S. Carpenter (ORCID: https://orcid.org/0000-0001-6775-6943)
- Aylin Z. Henstridge (ORCID: https://orcid.org/0000-0003-1517-8306)
- Tara L. Hogenson (ORCID: https://orcid.org/0000-0002-6246-6987)
- Mark Joseph Truty (ORCID: https://orcid.org/0000-0003-3776-527X)
- Peter Azorsa (ORCID: https://orcid.org/0000-0003-3953-1151)
- Molly Goldwasser (ORCID: https://orcid.org/0000-0001-8984-6157)
- Nicole Peterson (ORCID: https://orcid.org/0009-0000-6035-4124)
- Jonathan Y. Xia (ORCID: https://orcid.org/0000-0003-1225-2566)
- Filip Bednar (ORCID: https://orcid.org/0000-0001-5193-9817)
- Allison R. Schulman (ORCID: https://orcid.org/0000-0002-3913-8045)
- Padma Kadiyala (ORCID: https://orcid.org/0000-0002-3273-9905)
- Marina Pasca di Magliano (ORCID: https://orcid.org/0000-0001-9632-9035)
- Martín E. Fernández-Zapico (ORCID: https://orcid.org/0000-0002-8089-3907)
- Jorge D. Machicado (ORCID: https://orcid.org/0000-0002-2065-1367)
- Stacy B. Menees (ORCID: https://orcid.org/0000-0002-5891-4118)
- Vaibhav Sahai (ORCID: https://orcid.org/0000-0003-1892-1548)
- Jiaqi Shi (ORCID: https://orcid.org/0000-0003-4893-1587)
- Arvind Rao (ORCID: https://orcid.org/0000-0002-9613-426X)
- Costas Andreas Lyssiotis (ORCID: https://orcid.org/0000-0001-9309-6141)
- Erik‐Jan Wamsteker (ORCID: https://orcid.org/0009-0007-6689-1204)
- Timothy L. Frankel (ORCID: https://orcid.org/0000-0001-5987-0404)
- Nandini Arya (ORCID: https://orcid.org/0009-0002-2289-4423)
- Georgina Branch (ORCID: https://orcid.org/0009-0009-6270-3354)
Institutions
- Mayo Clinic (US)
- University of Michigan (US)
- Michigan Medicine (US)
- Mayo Clinic in Arizona (US)
- Mayo Clinic Hospital (US)
- Ann Arbor Center for Independent Living (US)
- Mayo Clinic in Florida (US)
Publication Details
- Journal
- Cancer Research
- Published
- 2026-09-21
- DOI
- https://doi.org/10.1158/0008-5472.can-26-0823
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00