Phytochemical screening, GCMS characterization and antioxidant studies of Methanolic extract of Cissus quadrangularis stem

ABSTRACTCissus quadrangularis L., belonging to the Vitaceae family, is a fast-growing perennialsucculent climber widely distributed in tropical and subtropical regions. It is commonlyknown as Hadjod, “Devil’s backbone,” or “Bone setter” due to its characteristic quadrangularstems and traditional use in bone healing. The study aimed to authenticate, extract andperform physicochemical and phytochemical evaluation of the methanolic extract of stem ofCissus quadrangularis.Methods: Pharmacognostic studies, phytochemical screening of the methanolic extract whichwas followed by GC-MS) studies for identification of phytoconstituents and its antioxidantpotential.Results: Phytochemical screening revealed presence of numerous phytoconstituents inmethanol extract. Of the 4 solvent extracts tested, percentage yield varied from methanolicextract (5.5±0.80), Ethyl acetate (4.0 ±0.24) and then in aqueous extract (3.8±0.75). GCMSprofile led to identification of methanolic extract as most potent extract. The antioxidantpotential also reinforced the results.Conclusions: We conclude that methanolic extract exhibits considerable antioxidant potentialand the findings provide a scientific basis for future pharmacological investigations owing torich presence of phytochemicals.