Isolation of a lipase-producing strain from yuncheng salt lake and enhancement of lipase activity via UV mutagenesis

{"Enhancing":[0],"lipase":[1,13,61,100,122,164,209,243,270],"activity":[2,183,213,234,244,271],"and":[3,9,18,47,65,75,87,92,98,105,126,151,180,201,220,272],"tolerance":[4,21],"can":[5],"help":[6],"address":[7],"energy":[8],"environmental":[10],"challenges.":[11],"Expanding":[12],"sources,":[14],"increasing":[15],"their":[16,20],"activity,":[17,123,210],"improving":[19],"under":[22],"various":[23],"conditions":[24,86],"are":[25],"urgently":[26],"needed.":[27],"A":[28],"water":[29],"sample":[30],"collected":[31],"from":[32,117],"Yuncheng":[33,118],"Salt":[34,119],"Lake":[35],"was":[36,51,124,127,144,149,156,171,177,184],"plated":[37],"using":[38],"the":[39,55,99,103,140,146,152,162,167,173,181,239,242,246],"\\"double-layer":[40],"plate\\"":[41],"method":[42],"to":[43,53,80],"isolate":[44],"\\"hard-to-culture\\"":[45],"microbes,":[46],"a":[48,131,192],"selective":[49],"medium":[50],"used":[52,79],"screen":[54],"isolated":[56,116],"strain":[57,114,204,248],"L5,":[58,115,166],"which":[59],"showed":[60,112],"activity.":[62,197],"Gram":[63],"staining":[64],"morphological":[66],"observation,":[67],"combined":[68],"with":[69,211,280],"16":[70],"S":[71],"rRNA":[72],"gene":[73],"sequencing":[74],"phylogenetic":[76],"analysis,":[77],"were":[78,96,108],"preliminarily":[81,128],"identify":[82],"this":[83],"isolate.":[84],"Culture":[85],"enzymatic":[88],"reaction":[89,175],"parameters":[90],"before":[91,259],"after":[93,235],"UV":[94,199,260,266],"mutagenesis":[95,200,261,267],"optimized,":[97],"activities":[101],"of":[102,142,165,245],"parent":[104],"mutant":[106,203],"strains":[107],"compared.":[109],"The":[110,135,223],"results":[111],"that":[113,258],"Lake,":[120],"possessed":[121],"Gram-positive,":[125],"identified":[129],"as":[130],"Bacillus":[132],"seohaeanensis-like":[133],"strain.":[134],"optimum":[136,147,168,174],"NaCl":[137,169],"concentration":[138,170],"for":[139],"growth":[141],"L5":[143,269],"15.0%,":[145],"pH":[148,187,221],"8.0,":[150],"highest":[153,182],"cell":[154],"density":[155],"reached":[157,250],"at":[158,186,215],"48":[159],"h.":[160],"For":[161],"crude":[163],"25.0%,":[172],"temperature":[176],"35.0":[178,218],"°C,":[179,219],"observed":[185],"8.0.":[188,222],"Trichloromethane":[189],"had":[190],"only":[191,227],"minor":[193],"effect":[194],"on":[195],"enzyme":[196,225],"After":[198],"screening,":[202],"L5M":[205,249],"exhibited":[206],"significantly":[207],"enhanced":[208],"maximum":[212],"achieved":[214],"25.0%":[216],"NaCl,":[217],"mutated":[224,247],"not":[226],"tolerated":[228],"trichloromethane":[229],"but":[230],"also":[231],"retained":[232],"appreciable":[233],"tris-aminomethane":[236],"treatment.":[237],"Under":[238],"optimized":[240],"conditions,":[241],"161.4":[251],"±":[252,263],"5.4":[253],"U/mL,":[254],"2.96-fold":[255],"higher":[256],"than":[257],"(54.6":[262],"4.7":[264],"U/mL).":[265],"improved":[268],"tolerance,":[273],"showing":[274],"better":[275],"application":[276],"prospects":[277],"in":[278],"environments":[279],"drastically":[281],"fluctuating":[282],"conditions.":[283]}