A compact, wireless fluorimeter system for continuous, real-time monitoring of tissue perfusion via in vivo fluorescence signals
In vivo fluorescence signals provide physiological and pathological insights for the interpretation of biological states associated with anatomical abnormalities or disease. Conventional methods for measuring fluorescence signals rely on bulky, costly instrumentation for imaging or for localized sensing. Here, we report a compact, wireless fluorimeter system for continuous, real-time measurements of the dynamics of fluorescence signals at locations of interest across the body. The approach represents a type of wearable that enables quantitative, interpretable fluorescence readouts and automatic data streaming to standard smart devices. The platform is compatible with injectable fluorescent species of medical relevance, such as indocyanine green, fluorescein, and methylene blue. Measurements from ischemic rodent and healthy porcine models, as well as those associated with limb amputation/replantation studies in rodents, demonstrate physiologically meaningful fluorescence profiles, with validation against ex situ blood analysis. These conceptual and technical advances offer the potential for convenient, continuous monitoring of fluorescence signals to support disease management and long-term patient care.
Authors
- John A. Rogers (ORCID: https://orcid.org/0000-0002-3830-5980)
- Igor R. Efimov (ORCID: https://orcid.org/0000-0002-1483-5039)
- Chanho Park (ORCID: https://orcid.org/0000-0002-7649-1315)
- Seonggwang Yoo (ORCID: https://orcid.org/0000-0002-9126-927X)
- Yonggang Huang (ORCID: https://orcid.org/0000-0002-0483-8359)
- Hak‐Young Ahn (ORCID: https://orcid.org/0000-0002-5441-1054)
- Kyeongha Kwon (ORCID: https://orcid.org/0000-0002-2373-7799)
- Seyong Oh (ORCID: https://orcid.org/0000-0002-3989-1117)
- Alan Nugent (ORCID: https://orcid.org/0000-0003-3535-8128)
- Tae Wan Park (ORCID: https://orcid.org/0000-0003-1137-5273)
- Woo‐Youl Maeng
- Haohui Zhang (ORCID: https://orcid.org/0000-0001-8432-3672)
- Min‐Seung Jo (ORCID: https://orcid.org/0000-0003-4486-7725)
- Anthony Banks (ORCID: https://orcid.org/0000-0002-0509-0370)
- Eric Rytkin (ORCID: https://orcid.org/0000-0003-2511-0655)
- Yalçın Külahçi (ORCID: https://orcid.org/0000-0002-6836-2732)
- Zeynep Demir
- Jamin Lee (ORCID: https://orcid.org/0000-0002-1832-0016)
- Lindsey Wade
- R. Janarthanan
- Seth D. Goldstein (ORCID: https://orcid.org/0000-0001-7806-4109)
- Bedreddin Sazoglu (ORCID: https://orcid.org/0009-0008-1613-2805)
- Tae Yeon Kim (ORCID: https://orcid.org/0000-0003-2440-9890)
- Vijay S. Gorantla (ORCID: https://orcid.org/0000-0003-0686-059X)
- Jong Uk Kim (ORCID: https://orcid.org/0000-0002-9683-7977)
- Alicia J. McLuckie (ORCID: https://orcid.org/0000-0003-0748-707X)
- Fatma Esra Demir
- Omer Dirican
- Kaiqing Zhang
- Andrew Pelech (ORCID: https://orcid.org/0009-0005-6211-6804)
- Peter Saba
Institutions
- Forest Institute (US)
- Inje University (KR)
- Northwestern University (US)
- Shirley Ryan AbilityLab (US)
- Korea Advanced Institute of Science and Technology (KR)
- Sejong University (KR)
- Northwestern University (PH)
- Lurie Children's Hospital (US)
- Neurological Surgery (US)
- Hanyang University (KR)
- Wake Forest University (US)
- Sungkyunkwan University (KR)
Publication Details
- Journal
- Science Advances
- Published
- 2026-09-18
- DOI
- https://doi.org/10.1126/sciadv.aeg8187
- Primary Topic
- Optical Imaging and Spectroscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministry of Education
- Ministry of Science, ICT and Future Planning
- Ministry of Science and ICT, South Korea
- National Institutes of Health