Protocol for collection and generation of bat primary lung and kidney cells
Some bat species host pathogenic viruses and represent recognized models for studying antiviral immunity. Here, we present a protocol for collecting and generating primary cells from wild bat tissues. We describe steps for tissue collection, enzymatic digestion, isolation, and cell expansion. We then detail procedures for immunocompetency testing of the established cultures. This protocol describes the generation of in vitro models for understudied non-model species, facilitating physiologically relevant assays to improve understanding of bat biology, immunology, and pathogen-host interactions.
Authors
- Daniel J. Becker (ORCID: https://orcid.org/0000-0003-4315-8628)
- Arinjay Banerjee (ORCID: https://orcid.org/0000-0002-2821-8357)
- Nancy B. Simmons (ORCID: https://orcid.org/0000-0001-8807-7499)
- Kaushal Baid (ORCID: https://orcid.org/0000-0002-4354-7941)
- Rita M. Quintela‐Tizon (ORCID: https://orcid.org/0000-0003-2382-3504)
- B. R. Ansil (ORCID: https://orcid.org/0000-0002-9787-4569)
- Amanda Vicente‐Santos (ORCID: https://orcid.org/0000-0001-6012-2059)
- Beckett L. Olbrys
- Manuela M.T. Pereira (ORCID: https://orcid.org/0009-0004-8992-6148)
Institutions
- American Museum of Natural History (US)
- University of British Columbia (CA)
- University of Waterloo (CA)
- University of Toronto (CA)
- University of Saskatchewan (CA)
- University of Oklahoma (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2026-09-17
- DOI
- https://doi.org/10.1016/j.xpro.2026.104838
- Primary Topic
- Bat Biology and Ecology Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Edward Mallinckrodt, Jr. Foundation
- Human Frontier Science Program
- Canada Research Chairs