Tail-degloving and cold storage facilitate primary cell culture from wild-caught rodents

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Institutions

Publication Details

Journal
Cryobiology
Published
2026-09-17
DOI
https://doi.org/10.1016/j.cryobiol.2026.105715
Primary Topic
Animal Ecology and Behavior Studies
Type
article
Field-Weighted Citation Impact
0.00

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article

Tail-degloving and cold storage facilitate primary cell culture from wild-caught rodents

Hiroyuki Imai, Goro Masuda, Sumito Matsuya, Takeshi Sasaki et al.
Cryobiology
Animal Ecology and Behavior Studies
article

Tail-degloving and cold storage facilitate primary cell culture from wild-caught rodents

Hiroyuki Imai, Goro Masuda, Sumito Matsuya, Takeshi Sasaki, Subaru Kageyama
article en

Abstract

The use of wild rodents as biological resources is hindered by the difficulties of live transport and primary culture contamination caused by skin bacteria. To address this, we developed a "tail-degloving" method to physically remove the contaminated external integument. Using the large Japanese field mouse (Apodemus speciosus), this method suppressed contamination compared to conventional biopsies, yielding cells within 5-7 days that were capable of subculturing and cryopreservation. The protocol withstood 4°C storage for up to 7 days and enabled contamination-free cell establishment from a large rodent. This tail-degloving protocol eliminates live transport risks and effectively establishes primary cells after a multi-day transport delay.

CryobiologyVol. 125
Kagoshima University (JP), Tokyo University of Agriculture (JP), Yamaguchi University (JP)
Asahi Glass Foundation
Openalex Percentile: Top 11%
Animal Ecology and Behavior Studies
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Tail-degloving and cold storage facilitate primary cell culture from wild-caught rodents — Hiroyuki Imai, Goro Masuda, et al. · Cryobiology (2026) | TGRS Research Map | TGRS