A TiO2@Au-Enhanced Portable Parallel Single-Electrode Electrochemiluminescence Array for High-Throughput Dual-Color Imaging Detection of Mycotoxins

Abstract A single-electrode electrochemical system (SEES) shows outstanding advantages of high-throughput detection and portable imaging. Yet, its traditional series structure makes it difficult for some luminescent materials to achieve clear electrochemiluminescence (ECL) imaging, and it also suffers from uneven voltage distribution. Thus, altering the SEES structure and enhancing ECL performance are key challenges to achieve high-resolution ECL imaging. Here, this study innovatively designed a unique parallel ten-channel SEES device, introduced HOF-14 and Agm/ATT-Au NCs as dual-color ECL luminophors, and used the coreactant accelerator TiO2@Au to enhance ECL of dual emitters for the first time, achieving spatially resolved high-throughput ECL imaging detection of dual mycotoxins. Compared with the traditional series structure, the parallel design in SEES effectively enhances the system’s voltage division effect, ensures the stability and consistency of each channel, and enables many emitters to achieve ECL imaging. The electrode structure was divided into two parts: Agm/ATT-Au NCs and HOF-14 were immobilized to the upper and lower parts, respectively. The present targets zearalenone (ZEN) and alternariol (AOH) introduced TiO2@Au to the electrode through an amplification strategy, effectively accelerating TPrA to generate more reactive radicals TPrA•+ for dual-emitters, achieving significant enhancement of dual-color ECL for fast and high-throughput imaging detection of ZEN and AOH. The ten-channel parallel design supports more emitters for simultaneous detection of multiple samples, significantly improving analysis efficiency. The enhanced ECL can be directly captured by a regular mobile phone without relying on large imaging equipment, providing a practical new strategy for on-site rapid screening and portable detection in analysis.