Minireview of the Peroxidase Mimic Catalytic Activity of Nanoparticles for Aptasensing of Antibiotics with NanoZymes

The prevalence of antibiotic residues in food and environmental matrices has intensified the need for rapid, sensitive, cost-effective, and portable analytical platforms capable of supporting on-site detection. Although conventional antibody-based sensors have shown high specificity, several limitations restrict the ability to create a trustworthy monitoring mechanism. As a better alternative, aptamer-based biosensors have emerged as promising alternatives owing to their high affinity, sensitivity, selectivity, and high chemical stability. Nowadays, aptamer-conjugated nanomaterials with enzyme-like activity have been demonstrated as highly sensitive and cost-effective aptasensing platforms. The present study comprehensively reviews the progress in aptasensing utilizing nanozymes for antibiotics detection with various transducer mechanisms, viz. colorimetric, electrochemical, fluorescent, and surface- enhanced Raman scattering (SERS). The review aims to provide a critical outlook on these nanozyme aptasensing platforms to highlight their potential with better sensitivity, portability and user-friendly applications in food safety and environmental monitoring.