In vivo efficacy of cefiderocol against ceftolozane/tazobactam- and ceftazidime/avibactam-resistant Pseudomonas aeruginosa carrying the AmpC E219K substitution

ABSTRACT Chromosomal AmpC variants selected during ceftolozane/tazobactam or ceftazidime/avibactam therapy may affect cefiderocol activity. We evaluated the impact of the E219K substitution on cefiderocol efficacy using isogenic Pseudomonas aeruginosa pairs in a murine bacteremia model. Cefiderocol achieved greater bacterial reductions in parental isolates than in E219K derivatives, despite susceptible MICs. Cefiderocol failed to achieve stasis against a cefiderocol-resistant isolate. E219K impaired cefiderocol efficacy in vivo , supporting close monitoring of cefiderocol activity against isolates carrying this variant.

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Journal
Antimicrobial Agents and Chemotherapy
Published
2026-09-16
DOI
https://doi.org/10.1128/aac.00724-26
Primary Topic
Antibiotic Resistance in Bacteria
Type
article
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article

In vivo efficacy of cefiderocol against ceftolozane/tazobactam- and ceftazidime/avibactam-resistant Pseudomonas aeruginosa carrying the AmpC E219K substitution

Pablo A. Fraile-Ribot, Biel Taltavull, Michelle Outeda-García, Juan Carlos Vázquez-Ucha et al.
Antimicrobial Agents and Chemotherapy
Antibiotic Resistance in Bacteria
article

In vivo efficacy of cefiderocol against ceftolozane/tazobactam- and ceftazidime/avibactam-resistant Pseudomonas aeruginosa carrying the AmpC E219K substitution

Pablo A. Fraile-Ribot, Biel Taltavull, Michelle Outeda-García, Juan Carlos Vázquez-Ucha, Alejandro Beceiro, Antonio Oliver, Andrea Muras, Jorge Arca-Suárez, Paula Guijarro-Sánchez, Isaac Alonso-García, Germán Bou, Lucía González-Pinto, Arianna Rodríguez-Coello, Andrea García-Pose, Gabriela A. Báez-Barroso
article en

Abstract

ABSTRACT Chromosomal AmpC variants selected during ceftolozane/tazobactam or ceftazidime/avibactam therapy may affect cefiderocol activity. We evaluated the impact of the E219K substitution on cefiderocol efficacy using isogenic Pseudomonas aeruginosa pairs in a murine bacteremia model. Cefiderocol achieved greater bacterial reductions in parental isolates than in E219K derivatives, despite susceptible MICs. Cefiderocol failed to achieve stasis against a cefiderocol-resistant isolate. E219K impaired cefiderocol efficacy in vivo , supporting close monitoring of cefiderocol activity against isolates carrying this variant.

Antimicrobial Agents and Chemotherapy
Universidade da Coruña (ES), Instituto de Salud Carlos III (ES), Health Research Institute of the Balearic Islands (ES), Complexo Hospitalario Universitario A Coruña (ES), Hospital Universitario Son Espases (ES), Instituto de Investigación Biomédica de A Coruña (ES)
Openalex Percentile: Top 19%
Antibiotic Resistance in Bacteria
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