Standardized Apis mellifera Propolis Extract (EPP-AF®) Reduces Egg Burden and Granulomatous Inflammation in Experimental Schistosoma mansoni Infection

Propolis has potential anthelmintic activity against Schistosoma mansoni, although its mechanisms remain poorly characterized. This study evaluated the biological effects of standardized Apis mellifera propolis extract (EPP-AF®) in experimental murine schistosomiasis. In vitro, EPP-AF® reduced adult worm viability and motility. In vivo, Swiss mice were allocated to four groups: EPP-AF® (4.5 mg/kg/day, orally, for 30 days), Praziquantel (PZQ; 50 mg/kg, days 45–51 post-infection), infected untreated control, and uninfected untreated SHAM control. EPP-AF® delayed oviposition and reduced egg production and tissue retention, granuloma formation, hepatic fibrosis, and eosinophilia. It also modulated splenic cytokine production, reducing inflammatory mediators and increasing IL-10. Molecular docking indicated favorable interactions between propolis phenolic compounds and SmCB1. Collectively, EPP-AF® exhibited antiparasitic and tissue-protective effects during experimental S. mansoni infection, supporting its pharmacological potential against schistosomiasis.

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Publication Details

Journal
Pathogens
Published
2026-09-15
DOI
https://doi.org/10.3390/pathogens15090980
Primary Topic
Bee Products Chemical Analysis
Type
article
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article

Standardized Apis mellifera Propolis Extract (EPP-AF®) Reduces Egg Burden and Granulomatous Inflammation in Experimental Schistosoma mansoni Infection

Josivan Regis Farias, João Gustavo Mendes Rodrigues, Alexandra Martins dos Santos Soares, Wallyson André dos Santos Bezerra et al.
Pathogens
Bee Products Chemical Analysis
article

Standardized Apis mellifera Propolis Extract (EPP-AF®) Reduces Egg Burden and Granulomatous Inflammation in Experimental Schistosoma mansoni Infection

Josivan Regis Farias, João Gustavo Mendes Rodrigues, Alexandra Martins dos Santos Soares, Wallyson André dos Santos Bezerra, Lucilene Amorim Silva, Vitor Augusto Ferreira dos Santos, Thiare S. Fortes, Sulayne Janayna Araújo Guimarães, Guilherme Silva Miranda, Irlla Correia Lima Licá, Mayara da Silva, Flávia Raquel Fernandes do Nascimento, Rosane Nassar Meireles Guerra, Ranielly Araújo Nogueira, Gleycka Cristine Carvalho Gomes Frazão, Maria Gabriela Sampaio Lira
article en

Abstract

Propolis has potential anthelmintic activity against Schistosoma mansoni, although its mechanisms remain poorly characterized. This study evaluated the biological effects of standardized Apis mellifera propolis extract (EPP-AF®) in experimental murine schistosomiasis. In vitro, EPP-AF® reduced adult worm viability and motility. In vivo, Swiss mice were allocated to four groups: EPP-AF® (4.5 mg/kg/day, orally, for 30 days), Praziquantel (PZQ; 50 mg/kg, days 45–51 post-infection), infected untreated control, and uninfected untreated SHAM control. EPP-AF® delayed oviposition and reduced egg production and tissue retention, granuloma formation, hepatic fibrosis, and eosinophilia. It also modulated splenic cytokine production, reducing inflammatory mediators and increasing IL-10. Molecular docking indicated favorable interactions between propolis phenolic compounds and SmCB1. Collectively, EPP-AF® exhibited antiparasitic and tissue-protective effects during experimental S. mansoni infection, supporting its pharmacological potential against schistosomiasis.

PathogensVol. 15(9)
Federal Institute of São Paulo (BR), Universidade Federal do Maranhão (BR)
Openalex Percentile: Top 11%
Bee Products Chemical Analysis
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