Photocatalytic Construction of Multivalent Peptide-Human Serum Albumin (HSA) Conjugates Capable of Effectively Attenuating Phagocytosis

{"Upon":[0],"entry":[1],"into":[2],"the":[3,11,25,67,73,124,135,140,177],"bloodstream,":[4],"nanocarriers":[5],"can":[6],"be":[7],"rapidly":[8],"cleared":[9],"by":[10,117,155],"mononuclear":[12],"phagocyte":[13],"system":[14],"(MPS),":[15],"limiting":[16],"their":[17],"clinical":[18],"translation.":[19],"In":[20,145],"this":[21],"study,":[22],"based":[23],"on":[24,72],"CD47-SIRPα":[26,136],"\\"don't":[27],"eat":[28],"me\\"":[29],"signaling":[30,137],"pathway,":[31],"we":[32],"functionalized":[33],"human":[34],"serum":[35],"albumin":[36],"(HSA)":[37],"with":[38,66,94,119,162],"peptides":[39],"(SP1)":[40],"truncated":[41],"from":[42,186],"CD47.":[43],"Ortho-nitrobenzyl":[44],"alcohol":[45],"(o-NBA)":[46],"was":[47,81],"used":[48],"as":[49],"a":[50,57,77,84,170],"photoreactive":[51],"handle":[52],"to":[53,62,121,164],"modify":[54],"SP1,":[55],"and":[56,102,130,157,172],"subsequent":[58],"photocatalysis":[59],"gave":[60],"rise":[61],"SP1-HSA":[63],"conjugates":[64,91,182],"(multi-HSA)":[65],"peptide":[68],"being":[69],"multivalently":[70],"displayed":[71],"albumin.":[74],"For":[75],"comparison,":[76],"monovalent":[78],"conjugate":[79],"(mono-HSA)":[80],"prepared":[82],"via":[83],"site-specific":[85],"thiol-maleimide":[86],"chemistry":[87],"at":[88],"Cys34.":[89],"Both":[90],"were":[92],"loaded":[93],"chlorin":[95],"e6":[96],"(Ce6)":[97],"for":[98,106,176,189],"in":[99,107],"vitro":[100],"evaluation":[101],"indocyanine":[103],"green":[104],"(ICG)":[105],"vivo":[108,146],"imaging.":[109],"We":[110],"found":[111],"that":[112,134,150],"multi-HSA@Ce6":[113],"reduced":[114,152],"macrophagic":[115,143],"uptake":[116,154],"∼8-fold":[118],"respect":[120,163],"mono-HSA@Ce6,":[122],"demonstrating":[123],"effectiveness":[125],"of":[126,142,179,184],"multivalency.":[127],"Competitive":[128],"binding":[129],"SIRPα-knockdown":[131],"assays":[132],"validated":[133],"pathway":[138],"mediated":[139],"attenuation":[141],"uptake.":[144],"biodistribution":[147],"studies":[148],"revealed":[149],"multi-HSA@ICG":[151],"hepatic":[153],"∼2-fold":[156],"significantly":[158],"prolonged":[159],"blood":[160],"circulation":[161],"unmodified":[165],"HSA@ICG.":[166],"This":[167],"work":[168],"establishes":[169],"facile":[171],"effective":[173],"photocatalytic":[174],"approach":[175],"construction":[178],"multivalent":[180],"peptide-protein":[181],"capable":[183],"escaping":[185],"phagocytic":[187],"clearance":[188],"drug":[190],"delivery":[191],"applications.":[192]}