Three-dimensional ultrastructural analysis of fertilization-defective Arabidopsis mutants using STEM and array tomography
{"Abstract":[0],"Double":[1],"fertilization":[2,132],"in":[3,138,198],"angiosperms":[4],"is":[5,196],"completed":[6],"by":[7],"the":[8,15,46,54,96,100,139,156,160,164,190,243],"fusion":[9,141],"of":[10,36,57,99,131,159,166,242],"two":[11],"sperm":[12,21,146,177,208],"cells":[13,147,178,209],"with":[14],"egg":[16,101,161,191],"and":[17,28,39,69,75,85,124,213,228],"central":[18],"cells.":[19],"While":[20],"membrane":[22,201,215,249],"proteins":[23],"such":[24],"as":[25,33,114],"GEX2,":[26],"DMP8/9,":[27],"GCS1/HAP2":[29],"have":[30,49],"been":[31],"identified":[32,89],"key":[34],"regulators":[35],"gamete":[37,127,140,236],"attachment":[38],"fusion,":[40],"their":[41],"specific":[42],"ultrastructural":[43],"roles":[44],"within":[45],"embryo":[47],"sac":[48],"remained":[50],"unclear":[51],"owing":[52],"to":[53,119,149,219,248],"technical":[55],"limitations":[56],"live-cell":[58],"imaging":[59],"based":[60],"on":[61],"fluorescent":[62],"markers.":[63],"Here,":[64],"we":[65],"optimized":[66],"high-pressure":[67],"freezing":[68],"freeze-substitution":[70],"protocols":[71],"for":[72,168,234],"Arabidopsis":[73],"pistils":[74],"performed":[76],"three-dimensional":[77],"reconstructions":[78],"using":[79],"scanning":[80],"transmission":[81],"electron":[82],"microscopy":[83],"(STEM)":[84],"array":[86,229],"tomography.":[87],"We":[88],"an":[90,239],"electron-dense,":[91],"partially":[92],"thickened":[93],"structure":[94,106],"at":[95,155],"apical":[97],"region":[98],"cell":[102],"before":[103,203],"pollination.":[104],"This":[105],"disappeared":[107],"following":[108],"wild-type":[109],"fertilization,":[110],"suggesting":[111,193],"it":[112],"serves":[113],"a":[115,185],"specialized":[116],"\\"docking":[117],"site\\"":[118],"accommodate":[120],"pollen":[121],"tube":[122],"contents":[123],"facilitate":[125],"subsequent":[126],"interactions.":[128],"Comparative":[129],"analysis":[130],"mutants":[133],"revealed":[134],"distinct":[135],"functional":[136],"stages":[137],"process.":[142],"In":[143,171],"gex2":[144],"mutants,":[145],"failed":[148,218],"establish":[150],"robust":[151],"connections":[152],"when":[153],"positioned":[154],"lateral":[157],"side":[158],"cell,":[162],"confirming":[163],"requirement":[165],"GEX2":[167],"initial":[169],"attachment.":[170],"contrast,":[172],"dmp8":[173],"dmp9":[174],"double":[175],"mutant":[176],"facing":[179],"each":[180],"other":[181],"were":[182],"observed,":[183],"exhibiting":[184],"more":[186],"distant":[187],"position":[188],"from":[189,245],"surface,":[192],"that":[194,226],"DMP8/9":[195],"involved":[197],"maintaining":[199],"stable":[200,246],"adhesion":[202,247],"fusion.":[204,222,250],"Furthermore,":[205],"although":[206],"gcs1":[207],"maintained":[210],"intimate":[211],"contact":[212],"displayed":[214],"thinning,":[216],"they":[217],"undergo":[220],"final":[221],"Our":[223],"findings":[224],"demonstrate":[225],"STEM":[227],"tomography":[230],"are":[231],"powerful":[232],"tools":[233],"visualizing":[235],"dynamics,":[237],"providing":[238],"integrated":[240],"model":[241],"transition":[244]}
Authors
- Seiji Takayama (ORCID: https://orcid.org/0000-0002-1432-487X)
- Tatsuya Awano (ORCID: https://orcid.org/0000-0002-4735-4506)
- Megumi Iwano (ORCID: https://orcid.org/0000-0002-5280-1649)
- Kaoru Mitsuoka (ORCID: https://orcid.org/0000-0003-1782-675X)
- Tomoko Igawa (ORCID: https://orcid.org/0000-0003-1829-6076)
- Fumiko Ishizuna (ORCID: https://orcid.org/0000-0003-0936-7098)
- Takayuki Kohchi (ORCID: https://orcid.org/0000-0002-9712-4872)
- Tatsuya Katsuno (ORCID: https://orcid.org/0000-0002-0395-4382)
- M. Horii
- Toshiyuki Mori (ORCID: https://orcid.org/0000-0001-8751-4562)
- Kazuhiro Aoyama
- Ari Yoshimura (ORCID: https://orcid.org/0009-0001-6281-5761)
- Hitomi Ichikawa
Institutions
- Chiba University (JP)
- Kyoto University (JP)
- National Center for Global Health and Medicine (JP)
- Thermo Fisher Scientific (Israel) (IL)
- The University of Tokyo (JP)
- Nara Institute of Science and Technology (JP)
- Tokyo Kasei Gakuin University (JP)
- The University of Osaka (JP)
Publication Details
- Journal
- PLANT PHYSIOLOGY
- Published
- 2026-09-14
- DOI
- https://doi.org/10.1093/plphys/kiag683
- Primary Topic
- Plant Reproductive Biology
- Type
- article
- Field-Weighted Citation Impact
- 0.00