Efficient genome editing in tomato using Cas12i3 variants
Systematic comparison of Cas12i gene-editing tools in tomato identifies Cas-SF01 as the most efficient variant, achieving editing efficiencies comparable to conventional Cas9 at some genomic sites and providing a compact, highly specific, and versatile tool for studying gene function, engineering fruit metabolism, and developing improved tomato varieties through precision breeding.
Authors
- Zhaobo Lang (ORCID: https://orcid.org/0000-0002-5551-0126)
- Siqun Wu
- Jian‐Kang Zhu (ORCID: https://orcid.org/0000-0001-5134-731X)
- Pengcheng Wang (ORCID: https://orcid.org/0000-0001-6043-4132)
- Bo Yu (ORCID: https://orcid.org/0000-0001-8964-7617)
- Meili Zhou
- Lei Zhu (ORCID: https://orcid.org/0000-0002-4359-3622)
- Jinkai Li
- Xiaomu Niu
Institutions
- BGI Group (China) (CN)
- Macau University of Science and Technology (MO)
- Southern University of Science and Technology (CN)
- Chinese Academy of Agricultural Engineering (CN)
- Center for Excellence in Molecular Plant Sciences (CN)
- Kai Biotech (South Korea) (KR)
- BGI Research (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2026-09-14
- DOI
- https://doi.org/10.1111/jipb.70391
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00