An isocaudomer-assisted AND-gate Cas12a biosensor for dual-gene identification of methicillin-resistant Staphylococcus aureus.
, discriminates MRSA from closely related pathogens, and demonstrates 100% concordance in samples from an artificial tilapia infection model. This enzyme-assisted, logic-controlled Cas12a strategy enhances specificity without requiring multiplexed crRNAs and simplifies reagents and workflow, offering a highly specific, programmable, multi-step biosensing strategy for the detection of multidrug- resistant bacteria.
Authors
- Yuanzhao Hu
- Xianyao Zhang (ORCID: https://orcid.org/0000-0002-2823-3485)
- Haimei Mao
- Hongliang Wang
- Changhua He
- Yingzi Lin
- Yi Wan
- Zhiqing Yang
Institutions
- Hainan University (CN)
- Hainan Provincial Academy of Agricultural Sciences (CN)
- Department of Ecology and Environment of Hainan Province (CN)
- Hainan Center for Disease Control & Prevention (CN)
Publication Details
- Journal
- PubMed
- Published
- 2026-09-14
- DOI
- https://doi.org/10.1039/d6an00541a
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00