Blood Sampling Techniques for Wild Native Chilean Rodents

Blood sampling is a critical component of wildlife research, particularly in studies of wildlife diseases. However, limited information exists on venipuncture sites for Chilean wild rodent species, such as the olive-colored akodont (Abrothrix olivaceus), Darwin's pericote (Phyllotis darwini), and degu (Octodon degus). Here, we describe field-tested venipuncture sites that allowed the collection of measurable blood volumes suitable for molecular detection of Trypanosoma cruzi in these species, based on fieldwork conducted in Chile's semiarid Mediterranean ecosystem. We provide anatomical illustrations and practical recommendations for non-lethal blood collection. This short communication aims to assist researchers in obtaining reliable blood samples while ensuring animal welfare and minimizing stress.

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Publication Details

Journal
Journal of Wildlife Diseases
Published
2026-11-04
DOI
https://doi.org/10.7589/jwd-d-25-00214
Primary Topic
Trypanosoma species research and implications
Type
article
Field-Weighted Citation Impact
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article

Blood Sampling Techniques for Wild Native Chilean Rodents

Carezza Botto‐Mahan, Natalia Lártiga, Antonella Bacigalupo, Juana Paola Correa et al.
Journal of Wildlife Diseases
Trypanosoma species research and implications
article

Blood Sampling Techniques for Wild Native Chilean Rodents

Carezza Botto‐Mahan, Natalia Lártiga, Antonella Bacigalupo, Juana Paola Correa, Juan Contardo, Andrea Yáñez-Meza, Esteban San Juan, Nicol Quiroga, Gemma Rojo, Rodrigo Salgado, Flavia Montenegro
article en

Abstract

Blood sampling is a critical component of wildlife research, particularly in studies of wildlife diseases. However, limited information exists on venipuncture sites for Chilean wild rodent species, such as the olive-colored akodont (Abrothrix olivaceus), Darwin's pericote (Phyllotis darwini), and degu (Octodon degus). Here, we describe field-tested venipuncture sites that allowed the collection of measurable blood volumes suitable for molecular detection of Trypanosoma cruzi in these species, based on fieldwork conducted in Chile's semiarid Mediterranean ecosystem. We provide anatomical illustrations and practical recommendations for non-lethal blood collection. This short communication aims to assist researchers in obtaining reliable blood samples while ensuring animal welfare and minimizing stress.

Journal of Wildlife DiseasesVol. 62(4)
Universidad de Santiago de Chile (CL), Instituto Butantan (BR), Universidad San Sebastián (CL), University of the Americas (CL), Universidad Santo Tomás (CL), University of O'Higgins (CL), University of Chile (CL)
Good health and well-being
Openalex Percentile: Top 34%
Trypanosoma species research and implications
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