Weak but repeated patterns of co-introgression of nuclear OXPHOS genes and mitochondrial DNA in Iberian wall lizards

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Publication Details

Journal
Genome Biology and Evolution
Published
2026-09-11
DOI
https://doi.org/10.1093/gbe/evag228
Primary Topic
Amphibian and Reptile Biology
Type
article
Field-Weighted Citation Impact
0.00
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article

Weak but repeated patterns of co-introgression of nuclear OXPHOS genes and mitochondrial DNA in Iberian wall lizards

Antigoni Kaliontzopoulou, Pierre‐André Crochet, João Pedro Marques, Gabriel Mochales‐Riaño et al.
Genome Biology and Evolution
Amphibian and Reptile Biology
article

Weak but repeated patterns of co-introgression of nuclear OXPHOS genes and mitochondrial DNA in Iberian wall lizards

Antigoni Kaliontzopoulou, Pierre‐André Crochet, João Pedro Marques, Gabriel Mochales‐Riaño, Catarina Pinho, Mathias LAIZE, Philippe Geniez, Aline Muyle
article en

Abstract

In this study, we took advantage of the previously reported instances of mitochondrial DNA capture in the Podarcis Iberian group, a speciose group of Iberian wall lizards, to test the hypothesis that nuclear genes from the OXPHOS (Oxidative phosphorylation) chain can co-introgress with the mitochondria as an evolutionary response to mitigate the costs of mitonuclear incompatibilities. Using dense population sampling and transcriptome data, we generated capture-sequence datasets for nuclear OXPHOS chain genes (nucOXPHOS), random nuclear loci (nucControl) and the complete mitochondrial genome. Phylogenetic analyses of nuclear and mitochondrial genes confirmed two previously identified events of mitochondrial introgression in the Podarcis Iberian group and revealed two new cases. Three of these cases have led to complete local mitochondrial DNA replacements, where the introgressed mitotypes have replaced the native ones in several populations, and involve a currently unknown and presumably extinct donor species, so-called "ghost lineage". Detecting introgression from ghost lineages, whose genomes are not accessible, remains challenging. To overcome this issue, we designed or adapted several tests aimed at detecting differential signals of introgression between our nucOXPHOS and nucControl gene sets. One of these tests, based on the effects of introgression on branch lengths in phylogenetic trees, uncovered a weak but consistently significant signal of partial co-introgression of nucOXPHOS genes compared to the genomic background (represented by the nucControl gene set) in three out of four cases of mtDNA introgression.

Genome Biology and Evolution
Centre National de la Recherche Scientifique (FR), Universidade do Porto (PT), Université de Montpellier (FR), Centre d'Écologie Fonctionnelle et Évolutive (FR), Institut de Recherche pour le Développement (FR), Universitat de Barcelona (ES)
Openalex Percentile: Top 14%
Amphibian and Reptile Biology
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