Dynamics of human cardiogenesis and its disruption in trisomy 21

Abstract Developmental dynamics involve the specification of diverse cell types and their spatial organization into multicellular niches 1 . Here we combine single-cell and spatial multiomics to define 21 distinct tissue niches in the developing heart, which we use to develop a context-aware, resolution-agnostic niche classification tool (TissueTypist). Applying high-resolution spatial profiling to the developing sinoatrial node, we resolve three pacemaker cell subtypes arrayed along a linear axis. First trimester subpopulations, such as pacemaker cells in the sinus horn and sinoatrial node head region, display neuroattractant programs and interact with parasympathetic neurons via interactions that include Eph–ephrin and semaphorin–plexin signalling. Temporal trajectories map the maturation of atrial and ventricular cardiomyocytes and uncover a lipid–metabolic switch and potential key regulators of cell-type identity. In the ventricle, we identify cellular and transcriptional gradients along both pseudotime and transmural axes, which provide molecular insights into myocardial compaction and maturation. Comparative profiling revealed that hearts with trisomy 21 are depleted in compact cardiomyocytes and exhibit increased apoptosis relative to euploid hearts. This finding was validated in isogenic-matched trisomy 21 and euploid cardiomyocytes derived from induced pluripotent stem cells. These early developmental perturbations may contribute to the increased risk of congenital heart disease associated with Down’s syndrome. In summary, we present a spatially resolved framework of human cardiac development to enable systematic explorations of developmental niches in health and disease.

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Publication Details

Journal
Nature
Published
2026-10-07
DOI
https://doi.org/10.1038/s41586-026-11125-y
Primary Topic
Congenital heart defects research
Type
article
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article

Dynamics of human cardiogenesis and its disruption in trisomy 21

Vincent R. Knight-Schrijver, Eva Lana‐Elola, Harriet F. Johnson, Laure Gambardella et al.
Nature
Congenital heart defects research
article

Dynamics of human cardiogenesis and its disruption in trisomy 21

Vincent R. Knight-Schrijver, Eva Lana‐Elola, Harriet F. Johnson, Laure Gambardella, Yizhou Yu, Kazumasa Kanemaru, Anna Wilbrey-Clark, Hongorzul Davaapil, Minal Patel, James J. Cranley, Siew Yen Ho, Sarah Amalia Teichmann, Anna Philpott, J. Patrick Pett, Rifdat Aoidi, Rebecca Hulbert, Sanjay Sinha, Laura Richardson, Noémie Combémorel, Kenny S. Roberts, Jore Van Wauwe, Victor L. J. Tybulewicz, Semih Bayraktar, Claudia I. Semprich, Xiaoling He, Nadav Yayon, Richard C. V. Tyser, Andreia Sofia Bernardo, Krzysztof Polański, Elizabeth J. Tuck, Ilaria Mulas, Jack A. Palmer, 이우찬, Shani Perera, John-Poul Ng-Blichfeldt, Monika Dabrowska, Rakeshlal Kapuge
article en

Abstract

Abstract Developmental dynamics involve the specification of diverse cell types and their spatial organization into multicellular niches 1 . Here we combine single-cell and spatial multiomics to define 21 distinct tissue niches in the developing heart, which we use to develop a context-aware, resolution-agnostic niche classification tool (TissueTypist). Applying high-resolution spatial profiling to the developing sinoatrial node, we resolve three pacemaker cell subtypes arrayed along a linear axis. First trimester subpopulations, such as pacemaker cells in the sinus horn and sinoatrial node head region, display neuroattractant programs and interact with parasympathetic neurons via interactions that include Eph–ephrin and semaphorin–plexin signalling. Temporal trajectories map the maturation of atrial and ventricular cardiomyocytes and uncover a lipid–metabolic switch and potential key regulators of cell-type identity. In the ventricle, we identify cellular and transcriptional gradients along both pseudotime and transmural axes, which provide molecular insights into myocardial compaction and maturation. Comparative profiling revealed that hearts with trisomy 21 are depleted in compact cardiomyocytes and exhibit increased apoptosis relative to euploid hearts. This finding was validated in isogenic-matched trisomy 21 and euploid cardiomyocytes derived from induced pluripotent stem cells. These early developmental perturbations may contribute to the increased risk of congenital heart disease associated with Down’s syndrome. In summary, we present a spatially resolved framework of human cardiac development to enable systematic explorations of developmental niches in health and disease.

Nature
Wellcome/MRC Cambridge Stem Cell Institute (GB), University of Cambridge (GB), The Francis Crick Institute (GB), Wellcome Sanger Institute (GB), Royal Brompton Hospital (GB), Imperial College London (GB)
Good health and well-being
Openalex Percentile: Top 33%
Congenital heart defects research
DOIOpen Access PDFOpenAlex
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