Injectable Alginate–Lysozyme–Tannic Acid Hydrogel with Antioxidant and Inflammation-Modulating Function: Laboratory Process Transfer and 3D Extrusion Printing

Oxidative stress and persistent inflammation can compromise tissue repair and the performance of locally delivered biomaterials. Here, we developed an aqueous alginate–lysozyme–tannic acid biohybrid hydrogel designed to combine injectability, antioxidant functionality, and extrusion-based printability. A composition screen varying alginate and lysozyme at a fixed tannic acid concentration identified a clear balance between flowability and structural integrity. An intermediate formulation provided shear-thinning and elastic-dominant rheological behavior, controlled hydration, cytocompatibility, antioxidant activity, and practical syringe/nozzle extrusion. The selected formulation was transferable to an approximately 80 mL batch and could be deposited into a multilayer structure. The findings are consistent with the formation of a cooperative polysaccharide–protein–polyphenol network and demonstrate the feasibility of integrating redox functionality with material processability. Future studies should focus on quantitative print fidelity validation, molecular interaction analysis, lysozyme activity retention, longer term stability, and application-relevant in vivo evaluation. Such development may support the future translation of injectable and printable antioxidant biomaterials for chronic wound management and tissue regeneration applications.