Soil Gene Sequencing in the Surrounding Area of DianShan Lake

This project focuses on the areas surrounding Dianshan Lake, an important ecological barrier and typical freshwater lake in the Yangtze River Delta region with prominent water environmental protection significance. To explore the internal mechanism of lake eutrophication, surface soil samples were systematically collected along three distinct environmental gradients, including near farmland, lake periphery, and near-shore zones. Three independent biological replicates were set for each sampling zone to guarantee the accuracy and statistical robustness of experimental data. By adopting high-throughput soil metagenomic sequencing technology and professional bioinformatic analysis methods, this project intends to systematically characterize the functional gene profiles that regulate nitrogen and phosphorus cycling processes in soil. It further evaluates the driving effect of soil nutrient cycling on lake eutrophication risks and puts forward scientific, targeted and zone-specific pollution mitigation and ecological restoration strategies.