Genomic characterization of the attenuated human cytomegalovirus strain TR-VAC developed for subviral particle vaccine production
We report the complete genome sequence of the attenuated human cytomegalovirus strain TR-VAC, developed for subviral particle vaccine production. Oxford Nanopore duplex sequencing confirmed all engineered modifications, including UL130 repair, UL25 stop codons, ddFKBP insertion, GFP deletion, and retention of the bacterial artificial chromosome backbone, without large-scale structural rearrangements.
Authors
- Nicole Büscher
- Matthias Linke (ORCID: https://orcid.org/0000-0002-7494-9812)
- Charlotte Hewel (ORCID: https://orcid.org/0000-0003-0967-0054)
- Bodo Plachter (ORCID: https://orcid.org/0000-0001-8388-2288)
- Hanno Schmidt (ORCID: https://orcid.org/0000-0001-8915-891X)
Institutions
- Johannes Gutenberg University Mainz (DE)
- University Medical Center of the Johannes Gutenberg University Mainz (DE)
Publication Details
- Journal
- Microbiology Resource Announcements
- Published
- 2026-09-10
- DOI
- https://doi.org/10.1128/mra.00397-26
- Primary Topic
- Cytomegalovirus and herpesvirus research
- Type
- article
- Field-Weighted Citation Impact
- 0.00