Base-pair resolution ATAC-seq during macrophage/neutrophil differentiation

Abstract Objectives The differentiation of cells during development and adult homeostasis requires extensive remodeling of the epigenome at multiple scales, from transcription factor binding sites (TFBS) to domains spanning hundreds of kilobases. We profiled the accessibility landscape of the genome and how it changes during the differentiation of an in vitro model of macrophage-neutrophil differentiation using the assay for transposase-accessible chromatin followed by sequencing (ATAC-seq) at high coverage. The dataset enables future studies of the dynamics of DNA accessibility during myeloid differentiation at the broader genome-wide level. Data description ATAC-seq was used to profile DNA accessibility in duplicate during the in vitro differentiation of a bipotential murine myeloid progenitor cell line, PUER, into macrophages and neutrophils at three and five timepoints respectively. The resulting libraries were sequenced to an average depth of ∼300 million reads per sample. The high depth of coverage and temporal sampling would allow the genome-scale analysis of accessibility dynamics during myeloid differentiation at the resolution of individual TFBS.