A study of ubiquitination and liquid-liquid phase separation related genes yields uba1 as a primary candidate biomarker for a role in multiple myeloma

Ubiquitination and liquid-liquid phase separation has been reported to be closely related to the development and occurrence of tumors, but its role in multiple myeloma (MM) remains unexplored. For the first time, based on ubiquitination and liquid-liquid phase separation prognosis related genes (ULLPSRGs), consistent clustering analysis and prognostic difference analysis were conducted, and a prognostic model was constructed through multivariate Cox regression. The model was validated on an independent dataset. Simultaneously conduct immune infiltration analysis, enrichment analysis and drug sensitivity evaluation. Then, the potential therapeutic targets of MM were confirmed through cell experiments. We have identified for the first time four ULLPSRGs associated with MM prognosis. Consensus cluster analysis based on four ULLPSRGs revealed that the two identified clusters had prognostic differences. A prognostic model was constructed based on ULLPSRGs and validated in an independent dataset. The signature is an independent risk factor for MM patients and significantly correlates with clinical factors, the signature was associated with the tumor microenvironment. Cell experiments have confirmed that inhibiting the expression of UBA1 can inhibit the cycle progression of MM cells and promote apoptosis. Our signature can provide clinicians with prognostic predictions and help guide the treatment of patients with MM.