Quantitative Visualization of Intracellular Nanometabolism Using Peak‐Shifted Dual‐State Emissive FRET Nanoprobes

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Publication Details

Journal
Advanced Science
Published
2026-09-06
DOI
https://doi.org/10.1002/advs.77509
Primary Topic
Luminescence and Fluorescent Materials
Type
article
Field-Weighted Citation Impact
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article

Quantitative Visualization of Intracellular Nanometabolism Using Peak‐Shifted Dual‐State Emissive FRET Nanoprobes

Atsushi Wakamiya, Daisuke Unabara, Ryuju Suzuki, Toru Nakazawa et al.
Advanced Science
Luminescence and Fluorescent Materials
article

Quantitative Visualization of Intracellular Nanometabolism Using Peak‐Shifted Dual‐State Emissive FRET Nanoprobes

Atsushi Wakamiya, Daisuke Unabara, Ryuju Suzuki, Toru Nakazawa, Hitoshi Kasai, Tasuku Hamaguchi, Hiroshi Uji‐i, Koji Yonekura, Yoshitaka Koseki, Akihide Hibara, Satoshi Katsube, Farsai Taemaitree, Keita Tanita, Jinwoo Sung, Kota Sato, Minsang Kim, Yutaro Miki, Kunikazu Ishii, Keisuke Kawakami
article en

Abstract

Many nanoscale materials, ranging from environmental particulates to carrier-free nanomedicine platforms, enter the human body, yet their metabolic fates remain poorly understood. This process involves complex physical changes and molecular transformations within lysosomes; however, the particle-to-molecule transition-defined here as "nanometabolism"-has remained difficult to quantify due to intrinsic limitations of nanoscale bioimaging. To circumvent the fluorescence quenching of conventional fluorophores and the challenges of observing nanoscale dynamics, peak-shifted, dual-state emissive nanoprobes composed entirely of donor-acceptor bithiophene dyes are introduced here. This approach establishes a highly adaptable design strategy for constructing heterogeneous organic nanoparticles templated by these molecular metrics. These molecular probes generate bright, ratiometric spectral shifts that directly encode nanoparticle disassembly, enabling robust optical monitoring of intracellular nanometabolism with cell-type- and particle-size-resolution. Time-resolved imaging identifies protonation-induced lysosomal membrane destabilization as the primary driver of nanoparticle breakdown within 6-12 h. Supported by cryogenic correlative imaging and tissue-level tracking, this platform provides a promising optical framework for monitoring nanometabolism in living systems. It serves as a pivotal breakthrough in elucidating the mechanistic design rules required for the future development of carrier-free nanomedicines with predictable intracellular behavior.

Advanced Science
Hokkaido University of Science (JP), Tokyo Institute of Technology (JP), Prefectural University of Hiroshima (JP), Tohoku University (JP), Kyoto University (JP), Sendai National College of Technology (JP), Tokyo Institute of Psychiatry (JP), Tohoku University Hospital (JP), SPring-8 (JP), KU Leuven (BE)
Openalex Percentile: Top 23%
Luminescence and Fluorescent Materials
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