ANALGESIC AND ANTI-INFLAMMATORY INVESTIGATION OF THIOPHENE MANNICH BASES IN RATS

Objective: The present investigation was undertaken to synthesize and systematically evaluate a series of thiophene mannich base derivatives for their analgesic and anti‑inflammatory potential using validated experimental models. The study aimed to assess both central and peripheral mechanisms of analgesia, as well as acute and chronic inflammatory responses.Material and Method: Algesia was induced using both thermal and chemical methods to evaluate central and peripheral analgesic effects, respectively. Inflammation was induced through peripheral models, namely carrageenan-induced paw edema, granuloma pouch, and formalin-induced paw edema. The test compounds were administered at a dosage of 55 mg/kg. Pentazocine (10 mg/kg, s.c.) served as the reference drug for the central analgesic model, whereas diclofenac (10 mg/kg) was used as the standard drug for peripheral analgesic and inflammatory models. Evaluation of analgesic and anti-inflammatory activity demonstrated that test compound 1, significantly prolonged response latency in the hot plate method, reduced the number of abdominal writhes induced by 1% acetic acid, decreased carrageenan- and formalin-induced paw edema, and reduced the volume of exudates in the air pouch model. Inflammatory markers such as Myloperoxidase enzyme, IL-6 and WBC counts were assessed.Result and Discussion: The test compound 1, has shown significant analgesic and anti-inflammatory activity.