Detection of Residual Pluripotent Stem Cells in Differentiated Cells—General Requirements for Probe‐Based Quantitative PCR Assay
Residual PSC detection follows ultra‑trace‑analysis principles. LOD supports qualitative assessment. Assays employ PSC‑specific markers and proper references, with adaptation to differentiated cell populations from diverse PSC sources.
Authors
- Boqiang Fu (ORCID: https://orcid.org/0000-0001-7035-8081)
- Qubo Chen (ORCID: https://orcid.org/0000-0003-2059-8217)
- Yu Alex Zhang (ORCID: https://orcid.org/0000-0001-7795-6252)
- Junying Yu
- Shijun Hu (ORCID: https://orcid.org/0000-0002-0068-8429)
- Zuliang Zheng
- 饶春明
- Tongbiao Zhao (ORCID: https://orcid.org/0000-0003-1429-5818)
- Jiani Cao (ORCID: https://orcid.org/0000-0001-8131-1297)
- Tao Na (ORCID: https://orcid.org/0000-0002-7564-2468)
- Yanhui Zhang (ORCID: https://orcid.org/0000-0001-7467-9106)
- Weiqi Zhang (ORCID: https://orcid.org/0000-0002-8885-5104)
- Aijin Ma (ORCID: https://orcid.org/0000-0003-1292-7025)
- Ranran Xu (ORCID: https://orcid.org/0000-0003-3060-4668)
- Glyn Stacey
- Shuyan Wang (ORCID: https://orcid.org/0009-0003-3764-3843)
- Lei Wang (ORCID: https://orcid.org/0009-0007-1837-1888)
- Tingting Zhang
- Siming Sun
- Yidi Gan
- Zijiang Yang
- Hao Wang
- Jian Xie
- Jie Hao
- Qiyuan Li
- Hongling Zhao
- Jun Wei
- Qiang Wei
- Cong Li
- Lihua Zhou
- Jing Fan (ORCID: https://orcid.org/0000-0002-9880-4503)
Institutions
- Guangzhou University of Chinese Medicine (CN)
- Beijing Technology and Business University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Beijing Institute of Genomics (CN)
- Soochow University (CN)
- National Institute of Measurement and Testing Technology (CN)
- National Institutes of Pharmaceutical Research and Development (China) (CN)
- Suzhou Research Institute (CN)
- Institute of Laboratory Animal Science (CN)
- National Institutes for Food and Drug Control (CN)
- Yebio Bioengineering (China) (CN)
- Kai Biotech (South Korea) (KR)
- BetaStem Therapeutics (United States) (US)
- Zephyr Software (United States) (US)
- Shenzhen Academy of Aerospace Technology (CN)
- Chinese Society for Electrical Engineering (CN)
- National Institute of Metrology (CN)
- University of Chinese Academy of Sciences (CN)
- Institute of Zoology (CN)
Publication Details
- Journal
- Cell Proliferation
- Published
- 2026-09-04
- DOI
- https://doi.org/10.1111/cpr.70276
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00