Branched-chain amino acid assimilation enables mixotrophy of ammonia-oxidizing archaeal sponge symbionts
Marine sponges and ammonia-oxidizing archaea (AOA) represent one of the earliest animal-microbe symbioses. AOA are considered metabolically constrained chemolithoautotrophs that remove nitrogenous waste within the sponge holobiont. Here, we expand this view by demonstrating that symbiotic AOA assimilate branched-chain amino acids (BCAA) as additional carbon and nitrogen sources. By combining stable isotope probing with fluorescence and chemical imaging, we trace the assimilation of 13 C- and 15 N-labeled BCAA (leucine, isoleucine, and valine) in the sponge holobiont Ianthella basta at single-cell resolution. We show that the ability to take up, degrade, and biosynthesize BCAA is a common adaptation among symbiotic AOA lineages. This ability may enable symbiotic AOA to modulate BCAA concentrations in their auxotrophic sponge hosts. Modulation of BCAA availability by symbionts may regulate the leucine-sensitive mTOR (mechanistic target of rapamycin) signaling pathway in sponges.
Authors
- Joana Séneca (ORCID: https://orcid.org/0000-0003-3951-3674)
- Stefan Schuster (ORCID: https://orcid.org/0000-0003-2828-9355)
- Margarete Watzka (ORCID: https://orcid.org/0000-0003-0334-1823)
- Bram Vekeman
- Michael Wagner (ORCID: https://orcid.org/0000-0002-9778-7684)
- Daan R. Speth (ORCID: https://orcid.org/0000-0002-2361-5935)
- Bettina Glasl (ORCID: https://orcid.org/0000-0002-6812-868X)
- Heidi M. Luter (ORCID: https://orcid.org/0000-0001-7810-7076)
- Erika Salas (ORCID: https://orcid.org/0000-0002-9917-8866)
- Katharina Kitzinger (ORCID: https://orcid.org/0000-0001-7382-7103)
- Wolfgang Wanek (ORCID: https://orcid.org/0000-0003-2178-8258)
- Petra Pjevac (ORCID: https://orcid.org/0000-0001-7344-302X)
- Anton A. Legin (ORCID: https://orcid.org/0000-0002-4088-5769)
- Katarina Damjanovic (ORCID: https://orcid.org/0000-0002-3893-4381)
- Nicole M. J. Geerlings (ORCID: https://orcid.org/0000-0003-2056-5650)
- Nathalie Heldwein (ORCID: https://orcid.org/0009-0009-6138-3782)
- Marie Rutsch (ORCID: https://orcid.org/0009-0006-4154-7119)
Institutions
- University of Vienna (AT)
- Australian Institute of Marine Science (AU)
- Medical University of Vienna (AT)
- Aalborg University (DK)
Publication Details
- Journal
- Science Advances
- Published
- 2026-09-04
- DOI
- https://doi.org/10.1126/sciadv.aef9450
- Primary Topic
- Marine Sponges and Natural Products
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Austrian Science Fund