Linker-Free Gold-Sputtered Tapered Optical Fiber Plasmonic Sensors for High-Sensitivity Refractive Index Detection in Microfluidic Platforms

Gold-sputtered tapered optical fiber (Au-TOF) sensors were developed for high-sensitivity refractive-index (RI) detection using aqueous glucose standards spanning 1.33–1.41 RIU (0–50% w/v). The sensors were fabricated using a reproducible workflow combining flame-brushing tapering, plasma surface preparation, and rotational magnetron sputtering, enabling azimuthally uniform gold coatings without the use of thiol or sulfur linker chemistries. Two sputtering durations (24 s and 30 s) were investigated to examine thickness-dependent plasmonic coupling and sensing performance. Optical measurements were conducted using a broadband supercontinuum source and compact spectrometer within a 20 µL microfluidic sensing chamber. Increasing glucose concentration produced a monotonic decrease in transmission intensity and a systematic red shift of the resonance minimum, consistent with enhanced evanescent-field interaction at the gold–dielectric interface. Across four independent trials, the 24 s Au-TOF sensor exhibited sensitivities up to 985 nm/RIU, while the 30 s device achieved sensitivities up to 1590 nm/RIU with strong linearity (R2 = 0.99). The enhanced sensitivity observed for the longer sputtering duration is attributed to improved gold film continuity and stronger plasmonic coupling. These results demonstrate a scalable, linker-free fabrication strategy for plasmonically enhanced tapered fiber sensors and establish the Au-TOF platform as a promising approach for label-free optical biosensing in compact microfluidic environments.